pMG36E
(Plasmid
#133853)
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Purpose(Empty Backbone) Expression vector
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133853 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepWV01
- Backbone size (bp) 1800
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Modifications to backboneEmR; mcs; P32
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Vector typeBacterial Expression
- Promoter P32
Growth in Bacteria
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Bacterial Resistance(s)Erythromycin, 200 μg/mL
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Growth Temperature30°C
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Growth Strain(s)E.coli MC100
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Growth instructionsThis plasmid can be propagated in a broad host range, including Lactococcus lactis, Escherichia coli, Bacillus subtilis and several Lactobacillus strains.
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMG36E was a gift from Jan Kok & Gerard Venema (Addgene plasmid # 133853 ; http://n2t.net/addgene:133853 ; RRID:Addgene_133853) -
For your References section:
Construction of a lactococcal expression vector: expression of hen egg white lysozyme in Lactococcus lactis subsp. lactis. van de Guchte M, van der Vossen JM, Kok J, Venema G. Appl Environ Microbiol. 1989 Jan;55(1):224-8. PubMed 2495760
Map uploaded by the depositor.
