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Plasmid#176856PurposeExpresses Ezrin fused with eGFP at the C-terminus in astrocytesDepositorInsertEzr-GFP
UseAAVTagsGFPExpressionBacterialMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Ubc13
Plasmid#18894DepositorInsertUbiquitin conjugating enzyme UBC13 (UBC13 Budding Yeast)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2008AvailabilityAcademic Institutions and Nonprofits only -
pHH03C_32
Plasmid#120897PurposeFunctional test of ECF sigma factors and their cognate promotersDepositorInsertECF32_1122
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcI-ts,ind+-(NSP8)
Plasmid#160656PurposeExpresses codon optimized SARS-CoV-2 NSP8 in E. coliDepositorInsertSARS-CoV-2 NSP8 (ORF1ab Synthetic)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 10, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNP1 sfGFP trigger
Plasmid#107359PurposeTrigger sequence for pNP1 sfGFP toehold switchDepositorInserttoehold trigger 7
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
4A3.SU
Plasmid#197675PurposeExpresses P1 S tail fibre and U chaperoneDepositorInsertsS
U
UseTagsExpressionBacterialMutationPromoterLate S promoter, LPS and N/AAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
4A3.S'U'
Plasmid#197676PurposeExpresses P1 S' tail fibre and U' chaperoneDepositorInsertsS'
U'
UseTagsExpressionBacterialMutationPromoterLate S promoter, LPS and N/AAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
SNX6-PX (27-171)
Plasmid#119087PurposeBacterial expression of human phox homology (PX) domain, SNX6-PX (27-171)DepositorInsertSNX6-PX (29-170) (SNX6 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceApril 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKEW107-MbCas12a-33362
Plasmid#115654PurposeExpresses MbCas12a in bacteria from M. bovoculi strain 33362, under TetR controlDepositorInsertMbCas12a
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceOct. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCJ207
Plasmid#162680PurposepET-21b(+) based plasmid for expression of the putative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with C-terminal His tag, codon optimized for expression in E. coli K12.DepositorInsertPutative MHETase from Hydrogenophaga sp. PML113 (Genbank WP_083293388.1) with signal peptide
UseTagsHisExpressionBacterialMutationcodon optimized for expression in E. coli K12PromoterT7/lacAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
MAPK3
Plasmid#73235PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMAPK3 (MAPK3 Human)
UseTagsHis-6-TEVExpressionBacterialMutationpfam00069:Pkinase 42-330PromoterT7Available sinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-tVYN-TmΔ
Plasmid#149694PurposeHigh-copy plasmid to express the tVYN-TmΔ luminescent biosensor for in vitro purificationDepositorInsertVenusΔC12-TmYcgRΔ-NLucΔ4
UseTagsExpressionBacterialMutationPromoterT7Available sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pASPIre3
Plasmid#154844PurposeDerivative of pASPIre2 lacking mCherry but containing a silent 150 bp DNA spacer flanked by attB/P sites; main uASPIre plasmid used in this studyDepositorInsertBxb1-sfGFP fusion controlled by rhamnose-inducible promoter; attB/attP-flanked silent discriminator
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pdUA-lacIN
Plasmid#141079PurposePlasmid vector for testing DNA binding proteins by the I-Block assay.DepositorTypeEmpty backboneUseVector for the i-block assay designed to study dn…TagsExpressionBacterialMutationPromoterEscherichia coli lacIN promoterAvailable sinceSept. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH04_32_sRNA-A_Sp_coD
Plasmid#120900Purposethe component plasmid of a regulated deviceDepositorInsertspECF32 promoter [i.e., Pecf32_1122 (-60 to -1)]
sRNA-A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAJM.714
Plasmid#108515PurposePVanCC-YFP Reporter. Van sensor for use in Marionette strain.DepositorInsertPVanCC-YFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Ptet-nCas9-BE
Plasmid#195739PurposeExpress APOVEC1-nCas9(D10A)-UGI using aTC inducible system in Methanotroph or E. coliDepositorArticleInsertTet repressor protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPtet(Tetracycline inducible protein)Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMP1335
Plasmid#119269Purposerfp "test" strainDepositorInsertdcas9
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
TDRD3 (3PMT)
Plasmid#28274DepositorInsertTudor domain-containing protein 3 (TDRD3 Human)
UseTagsHis with TEV cleavageExpressionBacterialMutationcontains Tudor domain, residues 553-611.PromoterAvailable sinceApril 14, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
PARP2-c098
Plasmid#216873PurposeBacterial expression vector for E. coli producing PARP2 catalytic protein (without Regulatory domain); with N-terminal CFP fusionDepositorInsertPARP2 catalytic domain (no Regulatory Domain) (PARP2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only