Skip to main content
Addgene

MAPK3
(Plasmid #73235)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 73235 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pNIC28-Bsa4
  • Backbone manufacturer
    SGC, Addgene Plasmid #26103
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    MAPK3
  • Alt name
    MAPK3A-c011
  • Alt name
    gi|38257141
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1234
  • Mutation
    pfam00069:Pkinase 42-330
  • Entrez Gene
    MAPK3 (a.k.a. ERK-1, ERK1, ERT2, HS44KDAP, HUMKER1A, P44ERK1, P44MAPK, PRKM3, p44-ERK1, p44-MAPK)
  • Promoter T7
  • Tag / Fusion Protein
    • His-6-TEV (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use BL21(DE3)-R3-lambda-PPase for protein expression. See also 4QTB: http://www.thesgc.org/structures/4QTB

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MAPK3 was a gift from Nicola Burgess-Brown (Addgene plasmid # 73235 ; http://n2t.net/addgene:73235 ; RRID:Addgene_73235)