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Plasmid#120543PurposeExpresses dFP biliverdin-binding fluorescence-activating mini 10 KDa protein in bacteria.DepositorInsertde novo fluorescence-activating protein mini (dFP-mini)
UseTags6xHis - TEV cleavageExpressionBacterialMutationPromoterT7Available sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBroad-T7RNAP-chlorR
Plasmid#199992PurposeT7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) chlorRDepositorInsertT7RNAP Circuit
UseTagsExpressionBacterialMutationcopFnullPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB744
Plasmid#176579PurposeExpressing the whole n-butanol pathway.DepositorInsertphaJ, ter, adhE2, phaA, phaB
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT1002
Plasmid#8621DepositorInsertbarnase, barstar
UseTagsas pMT416ExpressionBacterialMutationCI157 lambda repressor gene has two HindIII sites…PromoterAvailable sinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSLC-241
Plasmid#73194PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (mqsR) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertPrhaB-mqsR (mqsR )
UseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-E988K
Plasmid#173945PurposeBacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)DepositorInsertPARP1-E988K (PARP1 Human)
UseTags6xHisExpressionBacterialMutationV762A, E988KPromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-L713F
Plasmid#174794PurposeBacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationL713F, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-C125G
Plasmid#174792PurposeBacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)DepositorInsertPARP1-C125G (PARP1 Human)
UseTags6xHisExpressionBacterialMutationC125G, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM251 (miniCTX1-araC-ParaBAD)
Plasmid#110567PurposeIntegration vector for Pseudomonas aeruginosa with araC-ParaBAD inducible promoterDepositorInsertaraC-ParaBAD
UseTagsExpressionBacterialMutationPromoteraraC-ParaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Stat6-SH2
Plasmid#46517DepositorInsertStat6 (STAT6 Human)
UseTagsGST and PreScissionExpressionBacterialMutationcontains amino acids 510-654PromoterTacAvailable sinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJES103
Plasmid#135410PurposeE. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence under the control of hsp60 promoter that can be used as a reporterDepositorInsertblaTEM-1
UseTagsExpressionBacterialMutationE. coli 'blaTEM-1 with M. tuberculosis mpt63…Promoterhsp60Available sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM6-C4-mCherry
Plasmid#66530PurposeExpresses mCherry under the control of Mutant T7 Promoter C4DepositorInsertmCherry
UseSynthetic BiologyTagsExpressionBacterialMutationCodon Optimized for E. coliPromoterMutant T7 Promoter - C4Available sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Ec.coaE (pESC124)
Plasmid#50390PurposeHeterologous expression of E. coli CoA biosynthetic enzyme, DPCK (dephospho-coenzyme A kinase). Contain 6xHis-tag for nickel affinity purification.DepositorInsertDPCK
UseTags6XHIS tagExpressionBacterialMutationPromoterT7Available sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGST-PPARgamma
Plasmid#16549DepositorInsertPPAR gamma (PPARG Human)
UseTagsGSTExpressionBacterialMutationN-terminal DNA-binding domain: aa 1-248PromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
PARD3_PDZ_3
Plasmid#103959PurposeProtein expression and purification of PAR3 PDZ3 domainDepositorInsertPARD3 (PARD3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mEGFP-KanMX6
Plasmid#87023PurposeTag S. pombe genes in C terminal with mEGFPDepositorInsertmEGFP
UseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pMOD3-mClover-nat
Plasmid#120328PurposeBacterial mClover promoter trapDepositorInsertmClover
UseTagsExpressionBacterialMutationD103N (see comments below)Promoter(None)Available sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC319
Plasmid#61272PurposeAlso referred to as pΦRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA targeting the NDM-1 gene. Contains a ColE1 origin, kanamycin resistance cassette and f1 origin for packaging in M13 particles.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialMutationPromoterAvailable sinceApril 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-ffDronpa
Plasmid#78182PurposeExpresses ffDronpa E. coli strainsDepositorInsertffDronpa
UseTags6xHis-tag; Xpress tagExpressionBacterialMutationPromoterT7Available sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only