pRADZ1
(Plasmid
#47698)
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Purpose(Empty Backbone) General cloning vector for use in E. coli or D. radiodurans with lacZ
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 47698 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepI3 and pMTL23
- Backbone size (bp) 10000
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Modifications to backbonepRAD1 (pMTL23 carrying the SalI-FspI fragment of pI 12; 6.3kb.) carrying the lacZ fragment of pMUTIN2mcs; 10kb
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Vector typeBacterial Expression
- Promoter repU
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Cat-F
- 3′ sequencing primer LacZ-F (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRADZ1 was a gift from Mary Lidstrom (Addgene plasmid # 47698 ; http://n2t.net/addgene:47698 ; RRID:Addgene_47698) -
For your References section:
Characterization of the minimal replicon of a cryptic Deinococcus radiodurans SARK plasmid and development of versatile Escherichia coli-D. radiodurans shuttle vectors. Meima R, Lidstrom ME. Appl Environ Microbiol. 2000 Sep;66(9):3856-67. 10.1128/AEM.66.9.3856-3867.2000 PubMed 10966401