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Purpose(Empty Backbone) General cloning vector for use in E. coli or D. radiodurans
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 47697 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepI3 and pMTL23
- Backbone size (bp) 6280
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Modifications to backbonepMTL23 carrying the SalI-FspI fragment of pI 12; 6.3kb.
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Vector typeBacterial Expression
- Promoter repU
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Cat-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRAD1 was a gift from Mary Lidstrom (Addgene plasmid # 47697 ; http://n2t.net/addgene:47697 ; RRID:Addgene_47697) -
For your References section:
Characterization of the minimal replicon of a cryptic Deinococcus radiodurans SARK plasmid and development of versatile Escherichia coli-D. radiodurans shuttle vectors. Meima R, Lidstrom ME. Appl Environ Microbiol. 2000 Sep;66(9):3856-67. 10.1128/AEM.66.9.3856-3867.2000 PubMed 10966401