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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13421 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepACLU-Kan
- Backbone size w/o insert (bp) 3600
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Growth instructionsNeeds strain with lacIq
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameRNAP alpha-Gal4
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Insert Size (bp)900
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer AGA GCC TGA TAA AAA CGG TTA GCG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Expresses E. coli RNAP alpha subunit aa 1-248 fused to yeast Gal4 aa 58-97.
This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAC-Kan-alphaGal4 was a gift from Keith Joung (Addgene plasmid # 13421 ; http://n2t.net/addgene:13421 ; RRID:Addgene_13421) -
For your References section:
Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly. Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK. Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259 10.1038/nprot.2006.259 PubMed 17406455