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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 13480 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGP-FF
- Backbone size (bp) 3483
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL1 Blue
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Growth instructionsNeeds strain with lacIq
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNone
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Tag
/ Fusion Protein
- Gal11P (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BsgI (not destroyed)
- 5′ sequencing primer GGG GGA TTT CTG TTC ATG GGG G (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Bacterial cell-based
two-hybrid (B2H) expression vector. This plasmid is a corrected version of pGP-FB (Plasmid 13419).
Note that the BsgI site (not pictured in the map below) cuts at base 420.
pGP-FF requires a bacterial strain, such as XL1 Blue, that expresses the lacIq
allele of lac repressor. This ensures repression of the strong lacUV5 promoter,
which directs expression of the zinc finger-Gal11P hybrid protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGP-FF was a gift from Keith Joung (Addgene plasmid # 13480 ; http://n2t.net/addgene:13480 ; RRID:Addgene_13480) -
For your References section:
Standardized reagents and protocols for engineering zinc finger nucleases by modular assembly. Wright DA, Thibodeau-Beganny S, Sander JD, Winfrey RJ, Hirsh AS, Eichtinger M, Fu F, Porteus MH, Dobbs D, Voytas DF, Joung JK. Nat Protoc. 2006;1(3):1637-52. doi: 10.1038/nprot.2006.259 10.1038/nprot.2006.259 PubMed 17406455