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pTriEx-RhoA FLARE.sc Biosensor Q63L

Plasmid

#12151

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor Q63L (RHOA Human)

Use

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Mutation

RhoA Q63L (dominant-positive mutation)

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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XNOR-gate-V2.0

Plasmid

#44454

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

XNOR-gate

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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pDB-His-MBP-mGSDMD

Plasmid

#123365

Purpose

Expression of full-length mouse GSDMD in E. coli

Depositor

Hao Wu

Article

Orning et al Science. 2018 Nov 30;362(6418):1064-1069

Insert

GSDMD (Gsdmd Mouse)

Use

Tags

His6-MBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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Gi-c001

Plasmid

#173078

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GNAI1 (GNAI1 Human)

Use

Tags

mCitrine

Expression

Bacterial

Mutation

codon optimized

Promoter

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDD103

Plasmid

#119887

Purpose

Constitutive expression of mCherry on pAL5000/pMB1 backbone with kanamycin resistance.

Depositor

Tae Seok Moon

Article

DeLorenzo et al ACS Synth Biol. 2017 Oct 20;6(10):1973-1

Insert

pConstitutive_mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pETDuet-T7-Ct-WspR

Plasmid

#79166

Purpose

Dual expression of Ct biosensor and WspR WT under individual T7 promoters

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Inserts

WspR (wspR P. areuginosa)
T7-Spinach2-Ct

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX Stat5B-SH2

Plasmid

#46516

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Stat5B (STAT5B Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 593-692

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pET22b-mEB1-mCherry-6xHis

Plasmid

#130984

Purpose

Bacterial expression of mouse EB1-mCherry-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB1-mCherry-6xHis (Mapre1 Mouse)

Use

Tags

mCherry-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRSAN-QUE37C

Plasmid

#129312

Purpose

ATP measurement

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-huErCas12a

Plasmid

#174685

Purpose

Expresses 6xHis-MBP tagged ErCas12a

Depositor

Piyush Jain

Article

Nguyen et al medRxiv 2021.07.21

Insert

huErCas12a

Use

CRISPR and Synthetic Biology

Tags

6xHis, MBP, TEV, NLS and NLS, 6xHis

Expression

Bacterial

Mutation

Q926R mutation is observed as indicated from the …

Promoter

T7

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pNIC-HIS6-GST-TEV-GG

Plasmid

#215801

Purpose

Cloning of target gene with N terminal His6 GST tag and TEV cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEM-Cas9HF1

Plasmid

#89961

Purpose

Modified from pCas9-CR4 (Addgene: 62655) to use the high-fidelity version of Cas9, SpCas9-HF1 (N497A/R661A/Q695A/Q926A) from Kleinstiver et al 2016.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

Cas9HF1

Use

Tags

Expression

Bacterial

Mutation

N497A/R661A/Q695A/Q926A

Promoter

pTet

Available since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBAD_IPF2.0

Plasmid

#163124

Purpose

A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

Depositor

Julie Biteen

Article

Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

Insert

IFP2.0

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pOPINO_H11

Plasmid

#184277

Purpose

Expresses nanobody H11 in E. coli

Depositor

Ray Owens

Article

Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

Insert

Nanobody H11

Use

Tags

His6

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

T7

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pRM055 His-TEV-Cas9 6xNLS 0C

Plasmid

#196246

Purpose

Expression of NLS-rich Cas9 (0 Cys residues, mammalian codon optimized)

Depositor

Ross Wilson

Article

Foss et al Nat Biomed Eng. 2023 May;7(5):647-660. d

Insert

Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBbA13a-RFP

Plasmid

#126086

Purpose

Constitutive expression vector for Escherichia coli. BglBrick compatible.

Depositor

Nigel Scrutton

Article

Jervis et al ACS Synth Biol. 2019 Mar 18. doi: 10.102

Insert

RFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23102

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pMH48

Plasmid

#131861

Purpose

Produces holo-Cph1* in E. coli

Depositor

Wilfried Weber

Article

Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

Inserts

Cph1(1-514,Y263F)-RGD-His6-Cys
HO1
PcyA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter-1 and T7 promoter-2

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pZmUbi-5'UTR

Plasmid

#154057

Purpose

Level 0 Golden Gate vector, containing the ZmUbi promoter and 5' UTR with Level 0.5-compatible overhangs (Wheat construct)

Depositor

Cristobal Uauy

Article

Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

Insert

Promoter and 5' UTR Ubiquitin (Zea mays)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

N/A

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBNG2

Plasmid

#149473

Purpose

Broad-Host range expression vector, pBBR origin, NahR(AM) repressor, PSalTTC promoter, GFPmut3b, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Article

Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

Insert

NahR(AM)-GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PSalTTC

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST17-Cdc34a

Plasmid

#18674

Depositor

Wade Harper

Article

Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

Insert

Cdc34A (CDC34 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2008

Availability

Academic Institutions and Nonprofits only