pDB-His-MBP-mGSDMD
(Plasmid #123365)

• Purpose: Expression of full-length mouse GSDMD in E. coli
• Depositing Lab: Hao Wu
• Publication: Orning et al Science. 2018 Nov 30;362(6418):1064-1069. doi: 10.1126/science.aau2818. Epub 2018 Oct 25.

Backbone

• Vector backbone: pDB-His-MBP
• Backbone manufacturer: Berkeley Structural Genomics Center
• Backbone size w/o insert (bp): 6494
• Total vector size (bp): 7958
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: GSDMD
• Alt name: Gasdermin D
• Species: M. musculus (mouse)
• Insert Size (bp): 1464
• GenBank ID: NP_081236.1
• Entrez Gene: Gsdmd (a.k.a. 1810036L03Rik, DF5L, Dfna5l, GsdmD-1, Gsdmdc1, M2-4)
• Promoter: T7
• Tag / Fusion Protein:
  • His6-MBP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: CAGATGTCCGCTTTCTGGTATGCCG
• 3′ sequencing primer: GCTAGTTATTGCTCAGCGG

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry