30,260 results

1. pSB1A3 EcoRI-RTX with EcoRI Methylase-AmilCP

   Plasmid

   #85165

   Purpose

   Provides the coding region to express the EcoRI restriction enzyme. The RTX tag allows the EcoRI enzyme to be isolated via calcium-precipitation.

   Depositor

   Robin Dowell

   Article

   Hendrix et al ACS Synth Biol. 2014 Dec 19;3(12):969-71

   Insert

   EcoRI-RTX and EcoRI Methylase-AmilCP coding sequences
   

   Use

   Tags

   AmilCP and RTX

   Expression

   Bacterial

   Mutation

   Promoter

   J23100 promoter

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNF02-mLemon

   Plasmid

   #219399

   Purpose

   Constitutive expression of mLemonin E. coli

   Depositor

   Christian Lesterlin

   Article

   Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

   Insert

   mLemon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   proDp

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGTM_YR375_SUMO_Protease_001

   Plasmid

   #190063

   Purpose

   Expresses the yeast Ulp1 Sumo protease in active form as a TEV protease-cleavable MBP fusion

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   ULP1_YEAST (ULP1 Budding Yeast)
   

   Use

   Tags

   8X His-MBP (Maltose Binding Protein)

   Expression

   Bacterial

   Mutation

   Construct contains residues 347 – 621

   Promoter

   T7 lac

   Available since

   Jan. 30, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. SP-Cas9

   Plasmid

   #62731

   Purpose

   Expresses SP-Cas9 protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   SP-CAS9
   

   Use

   CRISPR

   Tags

   His tag and NLS

   Expression

   Bacterial

   Mutation

   Codon optimazed for e.coli

   Promoter

   T7

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. His6-SpyCatcher-mi3

   Plasmid

   #113043

   Purpose

   Expresses His6-SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

   Insert

   His6-SpyCatcher-mi3
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEVOL_PylRS(AF)-tRNA

   Plasmid

   #223511

   Purpose

   PylRS (AF)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

   Depositor

   Edward Lemke

   Article

   Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

   Inserts

   Pyrrolysyl-tRNA Synthetase (AF) (2 copies) (pylS Methanosarcina mazei)
   tRNA Pyl
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Y306A; Y384F

   Promoter

   araBAD, rrnB, and glnS and proK

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHEN2-SG4

   Plasmid

   #196071

   Purpose

   Express wild type SG4 in E.coli

   Depositor

   Shankar Balasubramanian

   Article

   Galli et al J Am Chem Soc. 2022 Dec 9. doi: 10.1021/

   Insert

   SG4
   

   Use

   Tags

   3XFLAG and 6XHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-MSP1D1delltaH5

   Plasmid

   #71714

   Purpose

   Membrane scaffold protein (MSP), helix 5 deletion

   Depositor

   Gerhard Wagner

   Article

   Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

   Insert

   Apolipoprotein A1 (APOA1 Human)
   

   Use

   Tags

   6xHis and TEV site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. ilux pGEX(-)

   Plasmid

   #107879

   Purpose

   Bacterial expression of ilux for bioluminescence imaging

   Depositor

   Stefan Hell

   Article

   Gregor et al Proc Natl Acad Sci U S A. 2018 Jan 30;11

   Insert

   ilux
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGLO-GFP-1UAG

    Plasmid

    #82500

    Purpose

    Expresses GFP with 1 UAG codon at the amino acid position 3

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Green Fluorescent Protein with 1 UAG codon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Added an UAG codon at the amino acid position 3

    Promoter

    AraBAD

    Available since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRM006

    Plasmid

    #98922

    Purpose

    Express Histag-SUMO-FtsZ for purification in E coli

    Depositor

    Jie Xiao

    Article

    Yang et al Science. 2017 Feb 17;355(6326):744-747.

    Insert

    FtsZ
    

    Use

    Tags

    Histag-SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. iYFP-Parkin Plasmid

    Plasmid

    #197107

    Purpose

    Expresses YFP-tagged Parkin when induced by Doxycycline in mammalian cells.

    Depositor

    Dori Woods

    Article

    Capelluto et al Adv Biol (Weinh). 2023 Jan 18:e2200246.

    Insert

    parkinson protein 2, E3 ubiquitin protein ligase (PRKN Human)
    

    Use

    Retroviral

    Tags

    YFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGLO-Blue

    Plasmid

    #220782

    Purpose

    Expresses a blue chromoprotein (visible light) with a his-tag and an inducible GFP (araBAD-promoter)

    Depositor

    Angela Leu

    Insert

    blue chromoprotein (aeBlue)
    

    Use

    Tags

    8xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMycVec2

    Plasmid

    #32361

    Depositor

    Michael Niederweis

    Article

    Kaps et al Gene. 2001 Oct 31;278(1-2):115-24.

    Insert

    pJAZ38 ori
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. TRBV7-9

    Plasmid

    #123437

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRBV7-9 (TRBV7-9 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. TRAV26-1

    Plasmid

    #123398

    Purpose

    construction of TCR

    Depositor

    Catherine Wu

    Article

    Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

    Insert

    TRAV26-1 (TRAV26-1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBS-hsFlt3L

    Plasmid

    #25074

    Depositor

    Maria Castro

    Article

    Curtin et al J Immunol. 2006 Mar 15. 176(6):3566-77.

    Insert

    human soluble FMS-like tyrosine kinase 3 ligand (FLT3LG Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. PdnaK GFP pZa

    Plasmid

    #170073

    Purpose

    GFP expression under the control of E. coli dnaK promoter

    Depositor

    Urartu Seker

    Article

    Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

    Insert

    Green fluorescent protein
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PdnaK

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGLO-Magenta-AZA

    Plasmid

    #220784

    Purpose

    Expresses a magenta chromoprotein (visible light and fluorescence) with a his-tag and an inducible GFP (araBAD-promoter)

    Depositor

    Angela Leu

    Insert

    magenta chromoprotein
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBeloBac11loxP2272

    Plasmid

    #60342

    Purpose

    This is a BAC vector that contains both wt and mutant loxP sites.

    Depositor

    Jon Coren

    Article

    Coren et al BMC Res Notes. 2017 Jul 28;10(1):344. do

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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