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Showing: 4261 - 4280 of 28150 results

  1. pKAR2-Br512g3.2

    Plasmid
    #171201
    Purpose
    Expresses Br512g3.2 polymerase in E.coli
    Depositor
    Andrew Ellington
    Article
    Bhadra et al medRxiv 2022.08.09.22278605
    Insert
    Engineered Bst DNA Polymerase I Large Fragment
    Use
    Tags
    8x His tag; Villin Headpiece 47 amino acid (vHP47…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    171201-plasmid-map-sequence-id-339558

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  4. pPP537

    Plasmid
    #158803
    Purpose
    pRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purification
    Depositor
    Jennifer Doudna
    Article
    Pausch et al Science. 2020 Jul 17;369(6501):333-337.
    Insert
    CasPhi-2
    Use
    Tags
    2xSV40NLS-6xHis
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158803-plasmid-map-sequence-id-309579

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  6. pGR

    Plasmid
    #46002
    Purpose
    Reporter plasmid for measuring terminator strength by inserting terminator of interest between the GFP and RFP, expression driven by a single PBAD promoter inducible by arabinose
    Depositor
    Christopher Voigt
    Article
    Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD
    Available since
    June 28, 2013
    Availability
    Academic Institutions and Nonprofits only
    46002-plasmid-map-sequence-id-64680

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  8. pDule2-Tet2.0

    Plasmid
    #85497
    Purpose
    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044
    Insert
    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G L65Q F108S Q109D D158S L162N
    Promoter
    lpp (constitutive)
    Available since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85497-plasmid-map-sequence-id-169854

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  11. PTPRK

    Plasmid
    #38968
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRK (PTPRK Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  14. pET28-MKH8SUMO

    Plasmid
    #79526
    Purpose
    Bacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Tags
    8xHis-Thrombin-SUMO-TEV and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2016
    Availability
    Academic Institutions and Nonprofits only

  18. pCRISPomyces-1

    Plasmid
    #61736
    Purpose
    Streptomyces expression of codon-optimized Cas9, tracrRNA, and custom crRNA
    Depositor
    Huimin Zhao
    Article
    Cobb et al ACS Synth Biol. 2014 Dec 8.
    Inserts
    sSpCas9
    tracrRNA
    crRNA cassette
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    BbsI-flanked lacZ cassette inserted in place of s…
    Promoter
    gapdhp(EL), rpsLp(CF), and rpsLp(XC)-BbsI
    Available since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only
    61736-plasmid-map-sequence-id-256988

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Showing: 4261 - 4280 of 28150 results