pNMT
(Plasmid #42578)

• Depositing Lab: James Hurley
• Publication: Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;109(42):16928-33. doi: 10.1073/pnas.1211759109. Epub 2012 Oct 1.

Backbone

• Vector backbone: pRSF-1
• Backbone manufacturer: Novagen/EMD Millipore
• Backbone size w/o insert (bp): 3600
• Total vector size (bp): 4968
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: N-myristoyl tranferase
• Alt name: NMT
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1368
• Mutation: two silent mutations T690C and T942C
• GenBank ID: M23726.1
• Entrez Gene: NMT1 (a.k.a. YLR195C, CDC72)
• Promoter: T7

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (unknown if destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7term

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Used for production of myristylated HIV-1 Gag in E. coli.