-
Plasmid#194759PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertTRIP12 (TRIP12 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceJuly 19, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
SNAP-23
Plasmid#186686PurposeExpresses SNAP-23 in E. ColiDepositorInsertSNAP23
UseTagsGST and thrombin siteExpressionBacterialMutationPromoterT7Available sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET His6 Sumo TEV expression vector with BioBrick polypromoter restriction sites (14-S)
Plasmid#48313DepositorTypeEmpty backboneUseTagsHis6 and SumoExpressionBacterialMutationPromoterAvailable sinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHBS834 H14-SUMO-TDP43 WT-TEV-mCherry
Plasmid#133320PurposeBacterial expression of TDP43 with WT CTDDepositorInsertTARDBP (TARDBP Human)
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceNov. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
EID1-pGex-2TK
Plasmid#22689DepositorInsertE1A-like inhibitor of differentiation 1 (EID1 Human)
UseTagsGSTExpressionBacterialMutationwt cDNAPromoterAvailable sinceDec. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAFi-dHdrA
Plasmid#184907Purposecarrying CRISPR/dCas9 system for gene knockdown in Acidithiobacillus ferriduransDepositorInsertCm-sgRNA-dCas9
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_PCNA_IRES_puro2b
Plasmid#21048DepositorInserthPCNA
UseTagsGFPExpressionBacterial and MammalianMutationPromoterAvailable sinceMay 15, 2009AvailabilityAcademic Institutions and Nonprofits only -
ASC-CASP1 Octamer
Plasmid#164032PurposeBacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamerDepositorUseTagsHis6-MBP-TEVExpressionBacterialMutationW169G (ASC), G20K (CASP1)PromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-rfp
Plasmid#153038PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array targeting the mrfp reporter gene.DepositorInsertAscpf1
UseTagsExpressionBacterialMutationE993APromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
-
pET-3c-rhVTN_FL
Plasmid#30223DepositorInsertrhVitronectin without deletions (VTN Human)
UseTagsExpressionBacterialMutationNo deletionsPromoterAvailable sinceMay 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
Traptavidin-E6
Plasmid#59549PurposepET21-Tre for expression of traptavidin (high stability mutant of streptavidin) bearing a glutamic acid tag for ion-exchange chromatographyDepositorInsertCore Traptavidin-E6
UseTagsE6 tagExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGCP123-GFP_g1
Plasmid#153518PurposesgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcodedDepositorInsertPnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP prescission LIC cloning vector (HMPKS)
Plasmid#29721DepositorTypeEmpty backboneUseTagsHis6-MBP-prescissionExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
CDC42
Plasmid#55693Purposeexpression of mouse CDC42DepositorInsertCDC42
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceSept. 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX 6P1 Cyclin A (555)
Plasmid#61844Purposebacterial expression of GST cyclin ADepositorInsertcyclin A (CCNA2 Human)
UseTagsGSTExpressionBacterialMutationL2VPromotertacAvailable sinceJune 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEB1-mCerulean3
Plasmid#103973PurposePlasmid encoding mCerulean3DepositorInsertmCerulean3
UseLow copyTagsExpressionBacterialMutationMutations relative to wild-type GFP (F64L, Y66W, …PromoterproCAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMRE142
Plasmid#118494Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertsGFP2
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPP537
Plasmid#158803PurposepRSF-Duet1 derived plasmid containing C-terminally 2xSV40 NLS and hexa-histidine tagged CasΦ-2 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-2
UseTags2xSV40NLS-6xHisExpressionBacterialMutationPromoterAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
6xHis(TEV)-Drp1-StrepII
Plasmid#174422PurposeExpresses human Drp1 Isoform 3 in bacteriaDepositorInsertDrp1
UseTags6xHis(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only