TRIP12-V2_A755-A841
(Plasmid
#194759)
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PurposeBacterial expression for structure determination; may not be full ORF
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 194759 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET28-MHL
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Backbone manufacturerSGC (Addgene Plasmid #26096)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTRIP12
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Alt namePDB: 7UW7, 8TRE
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Alt nameTRIP12:PBC037-C02:C247754
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SpeciesH. sapiens (human)
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Insert Size (bp)237
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Entrez GeneTRIP12 (a.k.a. MRD49, TRIP-12, TRIPC, ULF)
- Promoter T7
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Tag
/ Fusion Protein
- 6xHis (N terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7-term (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
PDB 7UW7: https://www.thesgc.org/structures/7uw7/
PDB 8TRE: https://www.rcsb.org/structure/8TRE/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TRIP12-V2_A755-A841 was a gift from Cheryl Arrowsmith (Addgene plasmid # 194759 ; http://n2t.net/addgene:194759 ; RRID:Addgene_194759)