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Showing: 3901 - 3920 of 28414 results

  3. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-StrepII (Human autophagy E3-like enzyme)

    Plasmid
    #169076
    Purpose
    Insect codon optimized ATG genes (excluded tags):10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-TEV-StrepII
    Depositor
    Sascha Martens
    Article
    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151
    Inserts
    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16 (ATG16L1 Human)
    Use
    Tags
    GGlinker, TEV cleavage site, StrepII and HIS10 Ta…
    Expression
    Bacterial and Insect
    Mutation
    Promoter
    Polyhedrin promoter
    Available since
    May 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    169076-plasmid-map-sequence-id-416841

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  4. pET15b LFn-Fla 3A

    Plasmid
    #84872
    Purpose
    Bacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.
    Depositor
    Russell Vance
    Article
    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi
    Inserts
    LFn
    Fla
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    3 C-terminal leucines have been replaced by alani…
    Promoter
    T7
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. pBRα

    Plasmid
    #53731
    Purpose
    Plpp/PlacUV5 -directed synthesis of E. coli RNAP alpha subunit; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53734.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    αNTD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    lpp/lacUV5 (tandem promoter)
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53731-plasmid-map-sequence-id-83909

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  7. EBFP2-pBAD

    Plasmid
    #54542
    Purpose
    Localization: Protein Expression Vector , Excitation: 383, Emission: 448
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Use
    Tags
    6xHis and EBFP2
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  8. WT-eGFP

    Plasmid
    #215168
    Purpose
    Encodes the enhanced green fluorescent protein
    Depositor
    Bhuvana Shanbhag, Travis Beddoe
    Insert
    WT-eGFP
    Use
    Tags
    His-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 lac
    Available since
    June 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    215168-plasmid-map-sequence-id-430164

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  16. p12x601

    Plasmid
    #157785
    Purpose
    EcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backbone
    Depositor
    Michael Rosen
    Article
    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi
    Insert
    pMD2.G(spei/spei)&12x601_tetO
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    157785-plasmid-map-sequence-id-357805

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  20. pYTK095

    Plasmid
    #65202
    Purpose
    Encodes AmpR-ColE1 as a Type 678 part to be used in the Dueber YTK system
    Depositor
    John Dueber
    Article
    Lee et al ACS Synth Biol. 2015 Apr 14.
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    65202-plasmid-map-sequence-id-110255

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Showing: 3901 - 3920 of 28414 results