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Genomic mining of prokaryotic repressors for orthogonal logic gates.

Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411. (Link opens in a new window) PubMed (Link opens in a new window) Article

Deposited by Christopher Voigt's lab, these response function plasmids (pRF-) contain a transcriptional repressor, which controls the expression of a YFP output. Repressors are under the control of the IPTG-inducible Ptac promoter. Expression of the YFP gene is controlled by a synthetic constitutive promoter that contains a repressor operator sequence. The YFP output is repressed in the presence of IPTG. The various circuit plasmids achieve either the NAND (pLux-LmrA and pNAND-PhlF) or AND (pAND) behavior. To generate the NAND behavior, the following input concentrations were used: no inducer (-/-), 1 mM IPTG (+/-), 20 µM 3OC6HSL (-/+), and 1 mM IPTG and 20 µM 3OC6HSL (+/+).To generate the AND behavior, the following inducer concentrations were used: no inducer (-/-), 1 mM IPTG (+/-), 100 ng/mL aTc (-/+), and 1 mM and 100 ng/mL aTc (+/+).

Voigt Lab Repressors for Orthogonal Logic Gates

Plasmids from Article

ID Plasmid Purpose
49355pRF-AmtR
49356pRF-AmeR
49357pRF-BetI
49358pRF-BM3R1
49359pRF-ButR
49360pRF-HapR
49361pRF-HlyIIR
49362pRF-IcaR
49363pRF-LitR
49364pRF-LmrA
49365pRF-McbR
49366pRF-Orf2
49367pRF-PhlF
49368pRF-PsrA
49369pRF-QacR
49370pRF-ScbR
49371pRF-SmcR
49372pRF-SrpR
49373pRF-TarA
49374pRF-TetR
49375pLux-LmrA
49376pNAND-PhlF
49377pANDExpression of AND gate in bacteria
51148pET21a-AcrR
51149pET21a-AmeR
51150pET21a-AmrR
51151pET21a-AmtR
51154pET21a-BarA
51155pET21a-BarB
51156pET21a-BetI
51157pET21a-BM1P1
51158pET21a-BM3R1
51159pET21a-BpeR
51161pET21a-CalR1
51162pET21a-CampR
51163pET21a-CasR
51164pET21a-CprB
51166pET21a-Cyp106
51167pET21a-DhaR
51168pET21a-Ef0113
51170pET21a-EthR
51172pET21a-HapR
51173pET21a-HemR
51174pET21a-HlyIIR
51176pET21a-IcaR-e
51177pET21a-IfeR
51178pET21a-JadR2
51179pET21a-KstR
51181pET21a-LitR
51183pET21a-LuxT
51184pET21a-McbR
51188pET21a-MtrR
51189pET21a-NonG
51191pET21a-Orf2
51192pET21a-orfL6
51193pET21a-PaaR
51195pET21a-PqrA
51210pET21a-TylP

Antibodies from Article