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Plasmid#119023PurposeE.coli expression plasmid for PmSPS1DepositorInsertPmSPS1
UseTags8xHisExpressionBacterialMutationPromoterT7Available sinceJune 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBSSK WT p85 alpha flag
Plasmid#13429DepositorInsertp85 alpha (PIK3R1 Human)
UseTagsflagExpressionBacterialMutationPromoterAvailable sinceNov. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
csr-1_hH1_mEos3.1_V5
Plasmid#191750PurposePhotoconvertible fluorescent nuclear reporter.DepositorInserthH1 (hH1 Neurospora crassa)
UseTagsV5, ccg-1 promoter, and mEos3.1ExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Cut-P(11)4
Plasmid#185785PurposeEncodes enzyme cutinase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertCut-P(11)4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACλCI
Plasmid#53730PurposePlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.DepositorInsertbacteriophage lambda CI protein
UseTagsExpressionBacterialMutationPromoterlac UV5Available sinceJune 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-GST-AGO3
Plasmid#24318DepositorInsertAGO3 (AGO3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-VAMP3
Plasmid#135552PurposeExpress rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertVAMP3
UseTags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-1-β4 hinge-ear
Plasmid#196692PurposeExpresses GST-AP-4 β4 hinge-ear (residues 534-739) in bacteriaDepositorInsertAP-4 β4 hinge-ear (534-739) (AP4B1 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQO18-TEV
Plasmid#27399DepositorInsertleuT
UseTagsHisX10 and TEVExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2011AvailabilityAcademic Institutions and Nonprofits only -
pSLC-237
Plasmid#73191PurposeTemplate plasmid which encodes kanamycin resistance gene for positive selection and toxin gene (relE) under the control of arabinose induceable promoter (ParaB) for negative selection.DepositorInsertParaB-relE
UseTagsExpressionBacterialMutationPromoterParaBAvailable sinceJune 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMSR
Plasmid#161983PurposeAllele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection markerDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTAMAHISTEV_PfeA
Plasmid#128943Purposeexpresses PfeA in E. coliDepositorInsertferric enterobactin receptor
UseTagsTEV protease cleavable 7xHis and TamAExpressionBacterialMutationSignal peptide sequence has been removed (1-75)PromoterT7Available sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCoofy49
Plasmid#55187PurposeBacterial expression vector for parallel SLIC cloning containing N-terminal 6x His and MBP tags. C-terminal tags can be added during SLIC.DepositorTypeEmpty backboneUseTags10x His-Stop-OneStrep-Stop-S-tag-Stop-CBP-Stop-HP…ExpressionBacterialMutationPromoterT7Available sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBXNPHM3
Plasmid#110099PurposeE.coli expression vector for FX cloning system, N-terminal pelB signal sequence followed by 10xHisTag, maltose binding protein and 3C cleavage siteDepositorTypeEmpty backboneUseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJLG039
Plasmid#192982PurposeBHR plasmid (IncP ori) expressing T7 polymerase under control of the lac promoterDepositorInsertT7 polymerase
UseTagsExpressionBacterialMutationPromoterlacAvailable sinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a SUMO Nup53xl 1-320
Plasmid#85998Purposebacterial expression of his6 and SUMO tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
UseTagsSUMOExpressionBacterialMutationcodon optimzed for bacterial expressionPromoterT7Available sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRDV5 containing Sb-convex
Plasmid#132696PurposepRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curveDepositorInsertSb_convex including 5' and 3' flanking sequences used for ribosome display
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-TRSter
Plasmid#78865PurposeExpresses rat Thioredoxin Reductase using an open reading frame in fusion with a mutated E. coli-type SECIS elementDepositorInsertThioredoxin Reductase (Txnrd1 Rat)
UseTagsMutated E. coli-type SECIS element (3’ end of ins…ExpressionBacterialMutationPromoterT7lacAvailable sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
6975 pPROEX-HTb-SIS1
Plasmid#1233DepositorInsertsis1 (SIS1 Budding Yeast)
UseTagshis tag (tev cleavable)ExpressionBacterialMutationPromoterAvailable sinceJuly 18, 2005AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-135
Plasmid#118521PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only