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Plasmid#48283DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only
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pHH0103_PRKACB_Pkinase
Plasmid#110024PurposeProtein expression and purification of PRKACB_PkinaseDepositorInsertPRKACB_Pkinase (PRKACB Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gαi2-LgB91
Plasmid#134358PurposeNanoluc complementation assay. Expression of Gαi2 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi2. Addition of the HA epitope at N terminus of Gαi2.DepositorInsertGαi2-LgB91 (GNAI2 Human)
UseTagsHAExpressionBacterial and MammalianMutationPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbdCas9S_P0526-sgRNAmreB
Plasmid#149654Purposeall-in-one CRISPRi vector for targeting B. burgdorferi mreBDepositorInsertdCas9, lacI, sgRNAmreB
UseCRISPRTagsExpressionBacterialMutationPromoterPflaB, PpQE30, P0526Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINO_NbH11-H4
Plasmid#155364PurposeExpresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2DepositorInsertH11-H4
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 4, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET_SZ1-RGG-GFP-RGG
Plasmid#124937PurposeExpression of SZ1-RGG-GFP-RGGDepositorInsertSYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBx-Spas-sgRNA-Kan
Plasmid#105233PurposePlasmid containing S. pasteurianus sgRNA with no 20bp target (Empty control) Kanamycin marker for P. putida and P. fluorescensDepositorInsertS. pasteurianus sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21-HIS-Tol2
Plasmid#112486Purposefunctional recombinant Tol2 protein from E.coliDepositorInsertTol2 Transposase (tpase Synthetic)
UseTagsHIS/6xExpressionBacterialMutationPromoterT7Available sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBLADE(FP6**)-mCherry
Plasmid#168049PurposeExpresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter. mCherry expression is controlled by the PBAD promoter.DepositorInsertsBlue light-inducible AraC dimers in Escherichia coli
mCherry
UseTagsExpressionBacterialMutationPromoterJ23101** and PBADAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 SARS-CoV 3CLpro
Plasmid#61692Purposeinducible expression of SARS 3CLpro proteaseDepositorInsertSARS 3CLpro protease
UseTagsGST and HISExpressionBacterialMutationPromotertacAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBAD miniSOG
Plasmid#91774PurposeBacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)DepositorInsertmini Singlet Oxygen Generator
UseTagsHexa-Histidine tagExpressionBacterialMutationPromoteraraBADAvailable sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCas-dCas12m
Plasmid#192275PurposeEncodes MmdCas12m (D485A) under a constitutive promoterDepositorInsertMmdCas12m
UseCRISPRTagsExpressionBacterialMutationD485APromoterPJ23108Available sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas9-beta
Plasmid#169240PurposeCas9 and beta expression plasmid for performing gene knockouts via oligo recombineeringDepositorInsertscas9
beta
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)
Plasmid#35152DepositorInsertAtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA
UseSynthetic BiologyTagsExpressionBacterialMutationR364S in Mevalonate KinasePromoterLacUV5, pTrcAvailable sinceMay 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMSP3545-dCas9Str
Plasmid#153516PurposeExpresses dCas9str under nisin-inducible nisA promoter in pMSP3545 backboneDepositorInsertdCas9Str
UseTagsExpressionBacterialMutationPromoternisAAvailable sinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-iRFP670
Plasmid#45453DepositorInsertiRFP670
UseTagsExpressionBacterialMutationT2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…PromoteraraBADAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNitET-SacB-kan
Plasmid#107692PurposeExpression of the phage Che9c RecET system for Recombineering in mycobacteriaDepositorInsertsChe9 phage RecET
PNit2-NitR
SacRB
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b 6His SpyCatcher-GRFT
Plasmid#204502PurposeBacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linkerDepositorInsertGRFT
UseSynthetic BiologyTags6xHis and SpyCatcherExpressionBacterialMutationPromoterT7Available sinceSept. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_ESRRG_Hormone-recep
Plasmid#109910PurposeProtein expression and purification of ESRRG_Hormone-recepDepositorInsertESRRG_Hormone-recep (ESRRG Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKEW302-AcrVA4
Plasmid#115656PurposeExpresses AcrVA4 in bacteria, under TetR control.DepositorInsertAcrVA4
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only