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PurposeBacterial expression of a fluorescent protein, mini Singlet Oxygen Generator (miniSOG)
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 91774 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD
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Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 4000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructions0.02% (weight/volume) of Arabinose is necessary for fluorescent protein production.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemini Singlet Oxygen Generator
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Alt nameminiSOG
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SpeciesA. thaliana (mustard weed); Evolved from Arabidopsis thaliana phototropin 2
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Insert Size (bp)321
- Promoter araBAD
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Tag
/ Fusion Protein
- Hexa-Histidine tag (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pBAD Forward
- 3′ sequencing primer unecessary (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
These plasmids are used in University Molecular Biology Laboratories and by High School students to purify fluorescent proteins and create fluorescent protein plate artwork.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBAD miniSOG was a gift from Erik Rodriguez & Roger Tsien (Addgene plasmid # 91774 ; http://n2t.net/addgene:91774 ; RRID:Addgene_91774) -
For your References section:
A genetically encoded tag for correlated light and electron microscopy of intact cells, tissues, and organisms. Shu X, Lev-Ram V, Deerinck TJ, Qi Y, Ramko EB, Davidson MW, Jin Y, Ellisman MH, Tsien RY. PLoS Biol. 2011 Apr;9(4):e1001041. doi: 10.1371/journal.pbio.1001041. Epub 2011 Apr 5. 10.1371/journal.pbio.1001041 PubMed 21483721