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Plasmid#127192PurposeProtein expression for affinity purificationDepositorInsertFUS 1-163 (FUS Human)
UseTagshexahistidineExpressionBacterialMutationPromoterAvailable sinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSL0527 (pDonor)
Plasmid#130634PurposeEncodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.DepositorInsertVchCAST donor DNA (Mini-Tn)
UseCRISPR; TransposonTagsExpressionBacterialMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEVA228-pro4IUPi
Plasmid#122018PurposeExpresses IUP pathway genes (ChK, IPK, idi) under constitutive promoter. Kan. resist.DepositorInsertsUseTagsExpressionBacterialMutationCodon optimized for E. coliPromoterpro4Available sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDIV296
Plasmid#204687PurposeAMA1 plasmid with nat1 resistance markerDepositorInsertnat1
UseFungal expressionTagsExpressionBacterialMutationPromoterAspergillus nidulans trpC promoterAvailable sinceDec. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCrhaB2
Plasmid#113634PurposeAn expression vector containing a rhamnose-inducible promoter to regulate gene expression in Burkholderia cenocepaciaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterPrhaBAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-HsRCK_E
Plasmid#146210PurposeBacterial Expression of HsRckDepositorInsertHsRck (DDX6 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD/HisB-iRFP720
Plasmid#45460DepositorInsertiRFP720
UseTagsExpressionBacterialMutationT2A, S13L, A92T, V104T, V114I, E161K, E180S, Y193…PromoteraraBADAvailable sinceJune 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBP-Pspac
Plasmid#112778PurposePromoter repressed by LacI (not natively produced in B. subtilis 168)DepositorArticleInsertPspac promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
mKate1.31-pBAD
Plasmid#54827PurposeLocalization: Protein Expression Vector , Excitation: 588, Emission: 635DepositorTypeEmpty backboneUseTags6xHis and mKate1.31ExpressionBacterialMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI
Plasmid#205162Purposeexpress scFv-GCN4-HAtag-msfGFP(V206K)-GBIDepositorInsertscFv-GCN4-HAtag-msfGFP(V206K)-GBI
UseTagsExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-3C-EmPNGaseF
Plasmid#218193PurposeBacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseFDepositorInsertPNGaseF
UseTagstufA-His-CBD-3CExpressionBacterialMutationcodon optimized Ecoli, residues 41-354PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5x_UnaG
Plasmid#163125PurposeA plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.DepositorInsertUnaG
UseTagsHis and MBPExpressionBacterialMutationPromoterAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSP573
Plasmid#98796PurposeExpression VectorDepositorInsertpET28 MBP-ENLYFQS-TEV WT
UseTagsExpressionBacterialMutationNonePromoterAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
SupD v2
Plasmid#198598PurposePlasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.DepositorInsertSupD
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOSIP-KC (KanR, PhiC31)
Plasmid#45982DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
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3xFLAG-dCas9/p-bacteria
Plasmid#64325PurposeExpresses 3xFLAG-dCas9 in E. coli in a tetracycline-dependent manner for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPR; Tetracycline-inducibleTags3xFLAGExpressionBacterialMutationD10A, H840APromoterpLtetO-1Available sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLG021 (AVAST type 5)
Plasmid#157899PurposeExpresses the AVAST type 5 defense systemDepositorInsertAVAST type 5 (STAND ATPase with N-terminal SIR2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDD108
Plasmid#119884PurposeIntegration vector into the ROCI-2 site of Rhodococcus opacus PD630 containing constitutive EYFP and hygromycin B resistance markerDepositorInsertBbaJ23104_EYFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -