pNRB150
(Plasmid
#36451)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 36451 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET-11a/pET-3a
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Backbone manufacturerNovagen
- Total vector size (bp) 6856
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDMC1
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneDMC1 (a.k.a. YER179W, ISC2)
- Promoter T7
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Tag
/ Fusion Protein
- 6His (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (destroyed during cloning)
- 3′ cloning site NdeI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See the attached maps for complete cloning details.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNRB150 was a gift from Douglas Bishop (Addgene plasmid # 36451 ; http://n2t.net/addgene:36451 ; RRID:Addgene_36451) -
For your References section:
Saccharomyces cerevisiae Dmc1 protein promotes renaturation of single-strand DNA (ssDNA) and assimilation of ssDNA into homologous super-coiled duplex DNA. Hong EL, Shinohara A, Bishop DK. J Biol Chem. 2001 Nov 9;276(45):41906-12. Epub 2001 Sep 10. 10.1074/jbc.M105563200 PubMed 11551925
Map uploaded by the depositor.
