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pOPINK-TRABID (OTU+AnkUBD, aa 245-697)
Plasmid#61590PurposeExpresses human TRABID (OTU+AnkUBD domains) in E. coli.DepositorInsertTRABID (ZRANB1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-244 and aa 698-708.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-pylT-AznLRS
Plasmid#172482PurposeFor E.Coli expression, 306L309L348I384F411WDepositorInsertAznLRS and pylTcua
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceJuly 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCR8-attL1-pegRNA-attR3
Plasmid#213051PurposeModular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3DepositorInsertsgRNA
UseTagsExpressionBacterialMutationPromoter35S-CmYCLV-AtU6Available sinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_3
Plasmid#78200PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, which also has a mutation to weaken phosphate binding, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,I76G,A197C)
UseTagsExpressionBacterialMutationA17C,I76G,A197C (please see depositor comment bel…PromoterT7Available sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA-wt_mScarlet-i_SGFP2
Plasmid#85071PurposeGreen/red FRET biosensor to monitor RhoA nucleotide loading state and activityDepositorInsertRhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)
UseTags6xHis and mScarlet-i and SGFP2ExpressionBacterial, Insect, and Mamm…MutationPromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
GST-Ub4
Plasmid#171421PurposeFor the purifcation of tetra linear ubiquitinDepositorInsertUbiquitin
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDFDuet-nsp7-nsp8
Plasmid#159092PurposeCoexpression construct of Nsp7 with an N-terminal His-tag and Nsp8DepositorInsertsNSP7
NSP8
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pComb3
Plasmid#63888PurposeFor phage display of antibody libraries by fusion to modified geneIII.DepositorTypeEmpty backboneUsePhage displayTagsgeneIIIExpressionBacterialMutationPromoterlacZAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAraGFPCDF
Plasmid#47516PurposeContains PBAD::GFP reporter, used for heterodimer competition assayDepositorInsertGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pENO1-dTom-NATr
Plasmid#52208PurposeConstitutive expression of Candida codon-optimized dTomato with NAT selectable markerDepositorInsertdTomato
UseTagsExpressionBacterial and YeastMutationcodon optimized for Candida speciesPromoterENO1Available sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSR43.6r
Plasmid#63197PurposeExpresses CcaS, Ho1, and PcyA constitutivelyDepositorInsertsCcaS
Ho1
PcyA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGP-RSET-iGABASnFR2-6xHis
Plasmid#218885PurposeBacterial expression of improved GABA sensor (positive change in fluorescence)DepositorInsertiGABASnFR2
UseTags6xHisExpressionBacterialMutationS99A F102Y F104Y L178SPromoterT7Available sinceMay 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-BirA*-dCas9-eGFP
Plasmid#84779PurposeExpresses BirA*-dCas9-eGFP in mammalian cells.DepositorInsertBirA*-dCas9-eGFP
UseCRISPRTagscMycExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceJuly 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
Parp14-c011
Plasmid#173098PurposeExpression of recombinant fusion protein in E. coliDepositorInsertPARP14 (PARP14 Human)
UseTagsmCeruleanExpressionBacterialMutationK791-F978PromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-CPDSalI
Plasmid#38252DepositorTypeEmpty backboneUseTags6xHis and Vibrio cholerae MARTX toxin cysteine pr…ExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pSEVA3b67Rb
Plasmid#58311PurposeShuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.DepositorInsertmRFP1
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28b-SpG-His
Plasmid#179318PurposePlasmid for bacterial expression and purification of SpG-Cas9DepositorInsertHuman codon-optimized Cas9 variant SpG
UseCRISPRTags6xHis and SV40-NLSExpressionBacterialMutationSpG=D1135L/S1136W/G1218K/E1219Q/ R1335Q/T1337RPromoterAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-Cezanne (OTU, aa 53-446)
Plasmid#61581PurposeExpresses human Cezanne (OTU domain) in E. coli.DepositorInsertCezanne (OTUD7B Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-52 and aa 447-843.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only
