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PurposeShuttle vector: contains constitutive mRFP1, compatible as reporter for E. coli/B. subtilis. See pSEVA3b61 for more info.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 58311 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSEVA3b61
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Growth instructionsIn E. coli, use chloramphenicol at 6 µg/mL (gene expression of cat not very strong). In B. subtilis, the same concentration is sufficient for selection.
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemRFP1
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SpeciesSynthetic
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For more info on shuttle vector backbone, see entry for pSEVA3b61.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSEVA3b67Rb was a gift from Tom Ellis (Addgene plasmid # 58311 ; http://n2t.net/addgene:58311 ; RRID:Addgene_58311) -
For your References section:
GeneGuard: a Modular Plasmid System Designed for Biosafety. Wright O, Delmans M, Stan GB, Ellis T. ACS Synth Biol. 2014 May 13. 10.1021/sb500234s PubMed 24847673