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pMC_MS2_DNA_VLP
Plasmid#179156PurposeExpression of maturation protein and his-tagged coat protein dimer from MS2 phageDepositorInsertsMaturation Protein
Coat Protein Dimer
UseTagsExpressionBacterialMutationPromoterT7Available sinceSept. 6, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFP.R267
Plasmid#138179PurposeExpresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE145
Plasmid#118497Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBADTrfp
Plasmid#99382PurposeThis promoter system provided the highest RFP expression upon inductionDepositorInsertRFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAW005-2
Plasmid#85611PurposeEmpty gRNA delivery vector for Bacillus subtilisDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGJJ001
Plasmid#183619PurposebindingPCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR under the expression of two CYC promoters.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCK255
Plasmid#171155PurposeExpression of I-SceI gene for genome engineering in Pseudomonas with sacB gene included for counterselection with sucrose on pBBR1-GmR plasmidDepositorInsertI-SceI_sacB
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-21(+)Tm9D8* TrpB
Plasmid#207517PurposeExpression of Tm9D8* TrpB for the synthesis of 7-Aza-L-Tryptophan (7AW) from 7-Azaindole (7AI) and serineDepositorInsertTryptophan synthase β-subunit
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK2-AraE
Plasmid#110141PurposeConstitutive promoter expressing araE on RK2 originDepositorInsertaraE
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEDA5_GFPmut3_Y66H
Plasmid#80085PurposePositive control plasmid for Nicking Mutagenesis. Constitutively expresses a non-fluorescent variant of GFPmut3 (Y66H) that is mutated to fluorescent (H66Y) in the protocol.DepositorInsertGFPmut3_Y66H
UseTags6x-His tagExpressionBacterialMutationchanged Tyrosine 66 to HistidinePromoterproBAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBinit
Plasmid#110100PurposeE.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal myc and 6xHisTagDepositorTypeEmpty backboneUseTagsmyc, HisExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAJM.336
Plasmid#108528PurposeLacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.DepositorInsertLacIAM + PTac-YFP
UseTagsExpressionBacterialMutationLacI Q18M A47V F161YPromoterAvailable sinceJuly 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDule2-pCNF
Plasmid#85495PurposeMachinery plasmid expressing permissive pCNF synthetase and cognate amber suppressing tRNA. Incorporates azidoPhenylalanine.DepositorInsertpara-cyanophenylalanine Mj synthetase
UseTagsNoneExpressionBacterialMutationY32L L65V F108W Q109M D158G I159PPromoterlpp (constitutive)Available sinceFeb. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPINE
Plasmid#26043DepositorInsertHis-lacZ-KHHHHHH
UseTagsKHHHHHH and MAHHHHHHSSGExpressionBacterial, Insect, and Mamm…MutationPromoterAvailable sinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHJL1
Plasmid#199376PurposeEncodes dual cistronic cytosolic GCaMP6s and mScarlet-I optimized for C. elegans expressionDepositorInsertGCaMP6s::SL2::mScarlet-I
UseTagsExpressionBacterialMutationno mutationsPromoterAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFVTd5
Plasmid#167520PurposeBroad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, tdKatushka2, Gentamicin Resistance MarkerDepositorInsertVanRAM-tdKatushka2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPVanCCAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSV272-PfMBP-HypDAE
Plasmid#110296PurposeExpresses t-4-hydroxy-L-proline dehydratase activating enzyme in E. coli BL21(DE3)DepositorInsertt-4-Hydroxy-L-proline Dehydratase Activating Enzyme
UseTagsHis6 and Maltose Binding ProteinExpressionBacterialMutationPromoterT7Available sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Xenopus (Silurana) tropicalis
Plasmid#75355PurposeNt His-tag Xenopus (Silurana) tropicalis Sacsin 87-1376DepositorInsertSacsin Xenopus (Silurana) tropicalis 87-1376
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAP84
Plasmid#86554PurposeExpresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficileDepositorInsertPveg-sLucopt
UseLuciferase and Synthetic BiologyTagsExpressionBacterialMutationPromoterPvegAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only
