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PurposeLacIAM + PTac-YFP Reporter. IPTG sensor for use in non-Marionette strain.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108528 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonep15A-Kan
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameLacIAM + PTac-YFP
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SpeciesSynthetic
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MutationLacI Q18M A47V F161Y
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/early/2018/04/10/285866 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAJM.336 was a gift from Christopher Voigt (Addgene plasmid # 108528 ; http://n2t.net/addgene:108528 ; RRID:Addgene_108528) -
For your References section:
Escherichia coli "Marionette" strains with 12 highly optimized small-molecule sensors. Meyer AJ, Segall-Shapiro TH, Glassey E, Zhang J, Voigt CA. Nat Chem Biol. 2018 Nov 26. pii: 10.1038/s41589-018-0168-3. doi: 10.1038/s41589-018-0168-3. 10.1038/s41589-018-0168-3 PubMed 30478458