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Syn61Δ3(ev5) ΔrecA (ev1)

Bacterial Strain

#189857

Purpose

Laboratory adapted, genomically recoded E. coli strain without TCG, TCA, or TAG codons and deleted serT, serU, prfA, and recA genes. Evolved for ~270 generations in LB at 37 °C.

Depositor

George Church

Article

Nyerges et al Nature. 2023 Mar;615(7953):720-727. doi:

Bacterial Resistance

Streptomycin

Species

Synthetic

Available since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only
HB101 endA::frt lambda pir

Bacterial Strain

#45472

Depositor

Michele Swanson

Article

Bryan et al Mol Microbiol. 2011 Apr;80(1):231-47. do

Bacterial Resistance

Streptomycin

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
ML2

Bacterial Strain

#61906

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. ilvE gene deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

Kanamycin

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF11

Bacterial Strain

#61961

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. metA gene deleted

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
RF6

Bacterial Strain

#62074

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. proC gene deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
B-95.ΔA

Bacterial Strain

#197933

Purpose

BL21(DE3)-based E. coli with 95 UAG codons and RF-1 being removed for translating UAG to noncanonical amino acids

Depositor

Kensaku Sakamoto

Article

Mukai et al Sci Rep. 2015 May 18;5:9699. doi: 10.103

Bacterial Resistance

None

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only
bSLS.114

Bacterial Strain

#191530

Purpose

Contains a knockout gene in the E. coli strain BL21-AI

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Bacterial Resistance

None

Species

Escherichia coli

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only
BL21 Rosetta2 ΔrecBCD

Bacterial Strain

#176583

Purpose

E. coli BL21 strain (with recBCD genomic locus deleted) for making cell-free extracts for Linear DNA expression. Contains pRARE2 plasmid.

Depositor

Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

Chloramphenicol

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain

Bacterial Strain

#99247

Purpose

LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain

Depositor

Jeff Hasty

Article

Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

Bacterial Resistance

Tetracycline

Available since

Nov. 1, 2017

Availability

Academic Institutions and Nonprofits only
RF13

Bacterial Strain

#62078

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
C321.deltaA (Isaacs lab)

Bacterial Strain

#73581

Purpose

Genomically recoded organism (E. coli C321.A, CP006698.1, GI:54981157). This strain is different than Addgene #68306 as it still retains the serB gene

Depositor

Farren Isaacs

Article

Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

Bacterial Resistance

Ampicillin

Available since

March 4, 2016

Availability

Academic Institutions and Nonprofits only
RF4

Bacterial Strain

#62072

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
HL 1255

Bacterial Strain

#52941

Purpose

MG1655 + fimS (off orientation)::gfp::Asp terminator::CamR at fimA

Depositor

Han Lim

Article

Hung et al Nat Commun. 2014 Aug 4;5:4574. doi: 10.1

Bacterial Resistance

Chloramphenicol

Species

Escherichia coli

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
C321.∆A T7RNAP

Bacterial Strain

#182776

Purpose

Recoded E. coli MG1655 strain with UAG termination function removed (RF1 is deleted); T7RNAP cassette genomically inserted

Depositor

Michael Jewett

Article

Perez et al Biotechnol J. 2021 Dec 11:e2100330. doi:

Bacterial Resistance

Spectinomycin

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only
BL21(DE3) ΔserC

Bacterial Strain

#197656

Purpose

Derivative of BL21(DE3) with the serC gene knocked out. This strain is used for expressing proteins containing site-specific non-hydrolyzable phosphoserine.

Depositor

Ryan Mehl, Richard Cooley

Article

Zhu et al ACS Cent Sci. 2023 Apr 10;9(4):816-835.

Bacterial Resistance

None

Available since

April 26, 2023

Availability

Academic Institutions and Nonprofits only
YM138

Bacterial Strain

#61953

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. cysE gene deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
EC1000

Bacterial Strain

#71852

Purpose

RepA+ MC1000, KmR , carrying a single copy of the pWV01 repA gene in the glgB gene; host for pOR128-based plasmids

Depositor

Todd Klaenhammer, Jan Kok

Article

Leenhouts et al Mol Gen Genet. 1996 Nov 27;253(1-2):217-

Bacterial Resistance

Kanamycin

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only
E. coli MEV15

Bacterial Strain

#197112

Purpose

E. coli BL21(DE3) derivative for sesquiterpenoid production with genome-integrated yeast MEV pathway, redox enzyme array, and Marionette cluster

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Bacterial Resistance

None

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only
CLM24 ∆lpxM

Bacterial Strain

#132780

Purpose

CLM24 strain with lpxM deletion

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

Bacterial Resistance

None

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only
MutaT7

Bacterial Strain

#156460

Purpose

An E. coli strain that expresses MutaT7 for mutagenizing genes downstream from a T7 promoter.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Bacterial Resistance

Streptomycin

Species

Rattus norvegicus

Available since

Oct. 19, 2020

Availability

Academic Institutions and Nonprofits only

Showing: 61 - 80 of 393 results

Results per page:

Sort results by:

Syn61Δ3(ev5) ΔrecA (ev1)

HB101 endA::frt lambda pir

ML2

RF11

RF6

B-95.ΔA

bSLS.114

BL21 Rosetta2 ΔrecBCD

LacZ deficient (lac operon deletion) BL21-Gold-dLac (DE3) strain

RF13

C321.deltaA (Isaacs lab)

RF4

HL 1255

C321.∆A T7RNAP

BL21(DE3) ΔserC

YM138

EC1000

E. coli MEV15

CLM24 ∆lpxM

MutaT7

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