Addgene

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Jun lab tunable CRISPRi strain

Bacterial Strain

#86400

Purpose

Strain SJ_XTL219 Genotype: MG1655 PBAD_dcas9 ΔlacI ΔaraE araFGH<>spec lacY A177C, galM<pBBa-J23119-tet-sacB-handle-term

Depositor

Suckjoon Jun

Article

Li et al Sci Rep. 2016 Dec 20;6:39076. doi: 10.10

Bacterial Resistance

Spectinomycin and hygromycin

Available since

Feb. 10, 2017

Availability

Academic Institutions and Nonprofits only
EcNR1 Strain

Bacterial Strain

#26930

Depositor

George Church

Article

Wang et al Nature. 2009 Aug 13. 460(7257):894-8.

Bacterial Resistance

Ampicillin

Available since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only
delta-Z

Bacterial Strain

#52706

Purpose

KIlac with lacZ deletion

Depositor

Tanja Kortemme

Article

Eames et al Science. 2012 May 18;336(6083):911-5. do

Bacterial Resistance

None

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only
39R861+

Bacterial Strain

#119737

Purpose

Six stable plasmids can be used as size standards

Depositor

Ruth Hall

Article

Moran et al Plasmid. 2019 Mar;102:6-9. doi: 10.1016/

Bacterial Resistance

None

Available since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only
BL21 ΔrecB

Bacterial Strain

#176580

Purpose

E. coli BL21 strain (with recB genomic locus deleted) for making cell-free extracts for Linear DNA expression.

Depositor

Chase Beisel, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Bacterial Resistance

None

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only
DH10B-ALT

Bacterial Strain

#61151

Purpose

DH10B modified to constitutively express araC, lacI, and tetR, integrated at the attB site on the E Coli genome. This is DH10B-ALT-Tet with the tetracycline resistance cassette deleted.

Depositor

Matthew Bennett

Article

Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

Bacterial Resistance

None

Available since

Oct. 2, 2015

Availability

Academic Institutions and Nonprofits only
EH1

Bacterial Strain

#102804

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC ilvA genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

Bacterial Resistance

None

Available since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only
EcM2.1.dtolC

Bacterial Strain

#64053

Purpose

Optimized strain for high-efficiency genome manipulations using CoS-MAGE and tolC cycling

Depositor

George Church

Article

Gregg et al Nucleic Acids Res. 2014 Apr;42(7):4779-9

Bacterial Resistance

Ampicillin

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
KJBAC1 strain

Bacterial Strain

#13424

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Bacterial Resistance

Tetracycline

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only
RF1

Bacterial Strain

#61962

Purpose

BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. glyA gene deleted

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods Enzymol. 2015;565:45-66. doi: 10

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
KIlac

Bacterial Strain

#52696

Purpose

MG1655 with Lac Operon removed from native locus and re-integrated at the attTn7 locus

Depositor

Tanja Kortemme

Article

Eames et al Science. 2012 May 18;336(6083):911-5. do

Bacterial Resistance

None

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only
DH10B-M.Osp807II

Bacterial Strain

#114267

Purpose

The strain expresses M.Osp807II methylase and zeocin selection marker from the arsB locus.

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Bacterial Resistance

Bleocin (zeocin)

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only
KJ740(DE3)

Bacterial Strain

#179486

Purpose

KJ740(DE3) is an OmpC-, OmpF-deletion strain of E. coli that allows for T7 promoter-based expression

Depositor

Debra Hansen

Article

Boyd et al FASEB J. 2022 Jul;36(7):e22378. doi: 10.

Bacterial Resistance

Tetracycline (chromosome, tn10) (10 μg/ml); streptomycin/spectinomycin (chromosome) (50 μg/ml)

Species

Escherichia coli

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only
ML17

Bacterial Strain

#61912

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. glnA gene deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
BL21ΔC

Bacterial Strain

#102258

Purpose

ompC deletion strain for overproduction of outer membrane proteins

Depositor

Jack Leo

Article

Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;

Bacterial Resistance

None

Available since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only
DH5alpha::DddI

Bacterial Strain

#179631

Purpose

For expression of toxic DddA

Depositor

David Liu

Article

Mok et al Nature. 2020 Jul 8. pii: 10.1038/s41586-

Bacterial Resistance

Gentamicin

Available since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only
E. coli ER1821ΔlacI

Bacterial Strain

#141407

Purpose

LacI-deficient E. coli host bacterium that produces β-galactosidase constitutively

Depositor

Antal Kiss

Article

Szentes et al Nucleic Acids Res. 2020 Mar 18;48(5):e28

Bacterial Resistance

None

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only
ML21

Bacterial Strain

#61914

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. hisG tyrA genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only
T-B18

Bacterial Strain

#159446

Purpose

E. coli BW25113 ΔfrmA, harboring plasmid Addgene plasmid # 129104, was adaptively evolved to grow efficiently on threonine and perform significant biosynthesis while employing methanol-derived carbon

Depositor

Maciek R. Antoniewicz, Eleftherios Terry Papoutsakis

Article

Har et al Appl Microbiol Biotechnol. 2021 Jan;105(

Bacterial Resistance

Ampicillin and kanamycin

Available since

Aug. 18, 2021

Availability

Industry, Academic Institutions, and Nonprofits
ML43

Bacterial Strain

#61922

Purpose

C43(DE3)-based E. coli amino acid auxotrophic host strains used for selective isotope labeling. cyo ilvE avtA aspC hisG argH metA lysA thrC asnA asnB genes deleted.

Depositor

Toshio Iwasaki, Robert Gennis

Article

Lin et al Methods. 2011 Dec;55(4):370-8. doi: 10.1

Bacterial Resistance

None

Available since

Jan. 26, 2015

Availability

Academic Institutions and Nonprofits only

Showing: 101 - 120 of 393 results

Results per page:

Sort results by:

Jun lab tunable CRISPRi strain

EcNR1 Strain

delta-Z

39R861+

BL21 ΔrecB

DH10B-ALT

EH1

EcM2.1.dtolC

KJBAC1 strain

RF1

KIlac

DH10B-M.Osp807II

KJ740(DE3)

ML17

BL21ΔC

DH5alpha::DddI

E. coli ER1821ΔlacI

ML21

T-B18

ML43

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