Addgene

We narrowed to 2,262 results for: PRK

Showing: 1921 - 1940 of 2262 results

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Anti-PICK1 [L20/8R]

Plasmid

#128630

Purpose

Mammalian Expression Plasmid of anti-PICK1 (Human). Derived from hybridoma L20/8.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-PICK1 (Human) recombinant mouse monoclonal antibody (PICK1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-MEK1 S218TAG

Plasmid

#68301

Purpose

N-terminal MBP tagged human MEK1 gene S218TAG for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 S218TAG (MAP2K1 Human, Synthetic)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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MBP-MEK1 S222TAG

Plasmid

#68302

Purpose

N-terminal MBP tagged human MEK1 gene S222TAG for bacterial expression

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

MBP-MEK1 S222TAG (MAP2K1 Human, Synthetic)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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YFP-Parkin C431N

Plasmid

#46924

Purpose

expresses mutant Parkin with YFP tag in mammalian cells

Depositor

Richard Youle

Article

Lazarou et al J Cell Biol. 2013 Jan 21;200(2):163-72.

Insert

Park2 (PRKN Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

changed cysteine 431 to asparagine

Promoter

CMV

Available since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 K177M

Plasmid

#73051

Purpose

Human AKT3 K177M

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 K177M (AKT3 Human)

Use

Tags

Expression

Mammalian

Mutation

K177M

Promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pEBG-MKK4-S255D/T259E (GST)

Plasmid

#21566

Depositor

Leonard Zon

Article

Sanchez et al Nature. . 372(6508):794-8.

Insert

mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Serine 255 to Aspartic Acid, Threonine 259 to Gl…

Promoter

Available since

July 17, 2009

Availability

Academic Institutions and Nonprofits only
pCIG AKT3 S472A

Plasmid

#73052

Purpose

Human AKT3 S472A

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 S472A (AKT3 Human)

Use

Tags

Expression

Mammalian

Mutation

S472A

Promoter

Available since

March 18, 2016

Availability

Academic Institutions and Nonprofits only

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139H2_HC

Plasmid

#206201

Purpose

For recombinant expression of the full heavy chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells. Includes a C-terminal -His8 tag for purification.

Depositor

Joost Snijder

Article

Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

Insert

anti-MUC1 antibody 139H2 heavy chain (Ighg1 Mouse)

Use

Tags

-AAAHHHHHHHH

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pEBG-MKK4-S255E/T259D (GST)

Plasmid

#21565

Depositor

Leonard Zon

Article

Sanchez et al Nature. . 372(6508):794-8.

Insert

mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Serine 255 to Glutamic Acid, Threonine 259 to As…

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pHH0103_MAP3K9-1/2

Plasmid

#91313

Purpose

Protein expression and purification of human SH3 domain construct MAP3K9-1/2

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

MAP3K9-1/2 (MAP3K9 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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mP58.dJ1-pCDNA3

Plasmid

#21884

Depositor

David Ron

Article

Oyadomari et al Cell. 2006 Aug 25. 126(4):727-39.

Insert

P58 (Dnajc3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

DnaJ domain deleted from C-term

Promoter

Available since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pWZLblasti-HA-MEK1 C121S

Plasmid

#53165

Purpose

Expresses HA tagged human MEK1 C121S from blasticidin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

C121S

Promoter

Available since

June 24, 2014

Availability

Academic Institutions and Nonprofits only
pCIG AKT3 S472E

Plasmid

#73053

Purpose

Human AKT3 S472E

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 S472E (AKT3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARF4-GFP

Plasmid

#67392

Purpose

Mammalian expression of hArf4 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

V53A, L123P and M134I mutations were unintended P…

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pPN186

Plasmid

#91573

Purpose

Express sgRNA targeting human AKT3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

AKT3 (AKT3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN187

Plasmid

#91574

Purpose

Express sgRNA targeting human AKT3

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

AKT3 (AKT3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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His 3C Kinase dead MKK6 1-334

Plasmid

#87882

Purpose

E. coli expression of MKK6 kinase dead

Depositor

Peter Mace

Article

Weijman et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

MKK6 (MAP2K6 Human)

Use

Tags

3C protease site and 6xHis

Expression

Bacterial

Mutation

K82A, kinase dead mutant

Promoter

T7

Available since

March 29, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-tetO-Mek1-shRNA

Plasmid

#53179

Purpose

Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (Map2k1 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2014

Availability

Academic Institutions and Nonprofits only
pGEX6P1-HA-MEK1 C121S

Plasmid

#53164

Purpose

Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector

Depositor

Christopher Counter

Article

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

MEK1 (MAP2K1 Human)

Use

Tags

GST and HA

Expression

Bacterial

Mutation

C121S

Promoter

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only
WNK1 gRNA (BRDN0001146391)

Plasmid

#75622

Purpose

3rd generation lentiviral gRNA plasmid targeting human WNK1

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

WNK1 (WNK1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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