Addgene

We narrowed to 11,119 results for: 110

Showing: 1601 - 1620 of 11119 results

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pNM3649

Plasmid

#154110

Purpose

miniMos dual component RMCE landing site vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

loxP::SEC::loxP::rpl-28p::FRT::GFP::his-58::unc-54 3'::FRT3

Use

Minimos

Tags

Expression

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)

Plasmid

#101105

Purpose

Encodes dCas9(C) (without VP64) fused to VEGFR1

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pHL661

Plasmid

#37635

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
mCherry

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1 and pLtetO-1

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only
pVM013

Plasmid

#64768

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, T7 to an HA-GFP tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Menendez-Benito et al Proc Natl Acad Sci U S A. 2013 Jan 2;110

Insert

RITE cassette

Use

Rite cassette

Tags

Expression

Mutation

Promoter

none

Available since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pGL2 enhancer- F3 LUC

Plasmid

#22429

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

F3 (LOC110973015 Human)

Use

Luciferase

Tags

luciferase reporter gene

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2010

Availability

Academic Institutions and Nonprofits only
pBDKRB2_TCS(Q’L)_HA-dCas9(N)_DHFR

Plasmid

#101109

Purpose

Encodes dCas9(N)-DHFR fused to BDKRB2

Depositor

Tudor Fulga

Article

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

BDKRB2-dCas9(N)-DHFR

Use

CRISPR and Synthetic Biology

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pGL2 enhancer- F2 LUC

Plasmid

#22428

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

F2 (LOC110973015 Human)

Use

Luciferase

Tags

luciferase reporter gene

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2010

Availability

Academic Institutions and Nonprofits only
pHL662

Plasmid

#37636

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

mCherry
GFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pLlacO-1 and pLtetO-1

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
GRM7

Plasmid

#28110

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

GRM7:DCC014-D04:C211307 (GRM7 Human)

Use

Tags

His tag with TEV site

Expression

Bacterial

Mutation

contains ligand binding domain, residues 37-513.

Promoter

Available since

March 18, 2011

Availability

Industry, Academic Institutions, and Nonprofits
pGL2 enhancer- F4 LUC

Plasmid

#22434

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

F4 (LOC110973015 Human)

Use

Luciferase

Tags

luciferase reporter gene

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2010

Availability

Academic Institutions and Nonprofits only
pGL2 enhancer- F5 LUC

Plasmid

#22436

Depositor

William Sessa

Article

Zhang et al J Biol Chem. 1995 Jun 23. 270(25):15320-

Insert

F5 (LOC110973015 Human)

Use

Luciferase

Tags

luciferase reporter gene

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2010

Availability

Academic Institutions and Nonprofits only
pLei-sfGFP-D134*

Plasmid

#191110

Purpose

An E. coli sfGFP reporter with one TAG at position 134

Depositor

Han Xiao

Article

Wu et al Protein Sci. 2022 Oct;31(10):e4443. doi:

Insert

superfolder green fluorescence protein

Use

Tags

His tag

Expression

Bacterial

Mutation

changing D134 to TAG

Promoter

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJBM2

Plasmid

#225289

Purpose

E. coli-Campylobacter shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pCC311 for pJBM2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pJBM3

Plasmid

#225290

Purpose

E. coli-Campylobacter shuttle vector; KanR

Depositor

Robert Moore

Article

McDonald et al mBio. 2024 Jul 29:e0110124. doi: 10.1128

Insert

Replication elements from pCC388 for pJBM3

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pNK887

Plasmid

#219728

Purpose

MoClo-compatible Level 0 - promoter vector carrying promoter MinSyn110

Depositor

Alexander Mishin

Article

Shakhova et al Int J Mol Sci. 2022 Dec 6;23(23):15441.

Insert

promoter MinSyn110

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

MinSyn110

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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PB-Blast-Dest-LifeAct-mRuby

Plasmid

#194907

Purpose

To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

LifeAct

Use

Mouse Targeting

Tags

mRuby

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pJBL6090

Plasmid

#193142

Purpose

Clostridium beijerinckii pfl ZTP riboswitch A26∆ OFF control with sfGFP reporter

Depositor

Julius Lucks

Article

Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

Insert

Cbe ZTP Riboswitch sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Deleted A26 to disable ligand-binding

Promoter

J23119

Available since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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YIplac211-SEC2-msGFPx3

Plasmid

#177110

Purpose

Expresses Sec2-msGFPx3 in yeast cells

Depositor

Benjamin Glick

Article

Casler et al J Cell Biol. 2022 Jan 3;221(1). pii: 212

Insert

SEC2 (SEC2 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)

Plasmid

#137110

Purpose

iFLinkC Linker cloning

Depositor

Viktor Stein

Article

Grawe et al Nucleic Acids Res. 2020 Jan 11. pii: 570

Insert

MBP-CS(TEV)-StrepTag-II-AI(TVMV)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pANTO3

Plasmid

#131107

Purpose

To generate a replicative plasmid carrying mycobacteriophage L5 integrase

Depositor

Riccardo Manganelli

Article

Boldrin et al Sci Rep. 2019 Apr 8;9(1):5783. doi: 10.1

Insert

mycobacteriophage L5 integrase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 18, 2019

Availability

Academic Institutions and Nonprofits only

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