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PB-Blast-Dest-LifeAct-mRuby
(Plasmid #194907)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 194907 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    PB/Blast-Dest
  • Backbone size w/o insert (bp) 7100
  • Total vector size (bp) 7939
  • Vector type
    Mammalian Expression, Mouse Targeting
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    LifeAct
  • Insert Size (bp)
    51
  • Tag / Fusion Protein
    • mRuby (C terminal on insert)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    LifeAct cDNA was taken from the plasmid published in Meng et al 2017: https://doi.org/10.1038/s41467-017-00977-8

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-Blast-Dest-LifeAct-mRuby was a gift from Clare Buckley (Addgene plasmid # 194907 ; http://n2t.net/addgene:194907 ; RRID:Addgene_194907)

  • For your References section:

    E-cadherin mediates apical membrane initiation site localisation during de novo polarisation of epithelial cavities. Liang X, Weberling A, Hii CY, Zernicka-Goetz M, Buckley CE. EMBO J. 2022 Aug 22:e111021. doi: 10.15252/embj.2022111021. 10.15252/embj.2022111021 PubMed 35993232
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