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pBABE puro human IRS-1
Plasmid#11025DepositorInsertIRS-1 (IRS1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (1-517, WT, MG-38-11)
Plasmid#110748PurposeTransient transfection of human USP30DepositorInsertUSP30 (USP30 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB-MeCP2
Plasmid#110821PurposeImproved dCas9 repressor-dCas9-KRAB-MeCP2DepositorInsertdCas9-KRAB-MeCP2
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Hyg)
Plasmid#110084PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
dCas9-KRAB
Plasmid#110820PurposeConventional dCas9 repressor-dCas9-KRABDepositorInsertdCas9-KRAB
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCMVAvailable sinceJuly 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-URH49 WT
Plasmid#110127PurposeExpresses Flag-tagged human URH49 (DDX39A)DepositorInsertURH49 (DDX39A Human)
UseTagsFlagExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceApril 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGM6
Plasmid#110660PurposeAdeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.DepositorInsertAAV6 cap genes, AAV2 rep genes, adenovirus helper genes
UseAAVTagsExpressionMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
STAT3-GFP
Plasmid#110495Purposereporter of STAT3 signaling activityDepositorInsert4 repeats of STAT3 binding site M67 at GFP promoter region
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW mCherry-GFP-LC3
Plasmid#110060PurposeTo visualize free autophagosomes (GFP and mCherry fluorescence) and autophagosomes that have fused with the lysosome (autolyosomes; mCherry fluorescence only, due to acid sensitivity of GFP)DepositorInsertmCherry-GFP-LC3
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMEV4
Plasmid#110539PurposeThe plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 28, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX330.puro
Plasmid#110403PurposeCRISPR/Cas9 vector with sgRNA expression and puromycin resistanceDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCBhAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRepCap6
Plasmid#110770PurposepRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.DepositorInsertAAV6 cap, AAV6 rep genes
UseTagsExpressionMutationPromoterAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-SOX4
Plasmid#110367PurposeMammalian expression of HA-tagged SOX4DepositorInsertSOX4 (SOX4 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3-OGDH_wobble
Plasmid#110938PurposeOGDH ORF containing silent wobble mutations insensitive to corresponding shRNAsDepositorInsertOGDH wobble cDNA (OGDH Human)
UseLentiviralTagsExpressionMutation15 silent wobble mutations corresponding to three…PromoterSV40Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shOGDH 2497
Plasmid#110942PurposeTet-inducible shRNA targeting OGDHDepositorInsertshOGDH (OGDH Human)
UseLentiviralTagsExpressionMutationPromoterH1/TOAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shOGDH 1714
Plasmid#110941PurposeTet-inducible shRNA targeting OGDHDepositorInsertshOGDH (OGDH Human)
UseRNAiTagsExpressionMutationPromoterH1/TOAvailable sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shOGDH 2956
Plasmid#110943PurposeTet-inducible shRNA targeting OGDHDepositorInsertshOGDH (OGDH Human)
UseLentiviral and RNAiTagsExpressionMutationPromoterH1/TOAvailable sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1D V5-His-TOPO-HuR.wt
Plasmid#110417PurposeMammalian expression of HuR (wild type)DepositorInsertHuR (ELAVL1) (ELAVL1 Human)
UseTags6xHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)
Plasmid#110559PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoterDepositorInsertrhaSR-PrhaBAD
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only
