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PurposeAdeno-Associated Virus (AAV) serotype 6; contains the AAV6 cap genes, AAV2 rep genes, and adenovirus helper genes. Good for in vivo transduction of muscle, lung, liver and other tissues.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 110660 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepDG
- Backbone size w/o insert (bp) 21845
- Total vector size (bp) 21842
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Modifications to backboneThe packaging/helper plasmid pDGM6, which includes the serotype 6 capsid reading frame, was generated by amplifying the capsid genefrom a clone of AAV serotype 6 by PCR using the oligonucleotide primers 5′- ATTTAAATCAGGTATGGCTGCCGATGGT-3′ and 5′- ATCGATTGCTATGGTGACCAGATAAGATAA-3′ encoding terminal SwaI and ClaI restriction sites and inserting the resulting DNA fragment into plasmid pDG cut with the same enzymes.
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Vector typeAAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha ; DH10B
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAAV6 cap genes, AAV2 rep genes, adenovirus helper genes
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Speciesadenovirus, adeno-associated virus
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SwaI (not destroyed)
- 3′ cloning site ClaI (not destroyed)
- 5′ sequencing primer unknown
- 3′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDGM6 was a gift from David Russell (Addgene plasmid # 110660 ; http://n2t.net/addgene:110660 ; RRID:Addgene_110660) -
For your References section:
Systemic delivery of genes to striated muscles using adeno-associated viral vectors. Gregorevic P, Blankinship MJ, Allen JM, Crawford RW, Meuse L, Miller DG, Russell DW, Chamberlain JS. Nat Med. 2004 Aug;10(8):828-34. doi: 10.1038/nm1085. Epub 2004 Jul 25. 10.1038/nm1085 PubMed 15273747