pHL662
(Plasmid
#37636)
-
Depositing Lab
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 37636 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepZ with p15a origin
-
Backbone manufacturerLim Lab
-
Modifications to backbonePlasmid modified from Rolf Lutz and Hermann Bujard, 1997
-
Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)XL1 Blue
-
Copy numberHigh Copy
Gene/Insert 1
-
Gene/Insert namemCherry
-
Insert Size (bp)711
- Promoter pLtetO-1
Cloning Information for Gene/Insert 1
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site ApaI (not destroyed)
- 5′ sequencing primer CAGTCATAGCCGAATAGCCT
- 3′ sequencing primer GTA TGT TGC ATC ACC TTC ACC CTC TCC ACT GAC AG (Common Sequencing Primers)
Gene/Insert 2
-
Gene/Insert nameGFP
-
Insert Size (bp)717
- Promoter pLlacO-1
Cloning Information for Gene/Insert 2
- Cloning method Restriction Enzyme
- 5′ cloning site XmaI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer GACGGGCCC ATGGTACGCGTGCTAGAGG
- 3′ sequencing primer ccggaattc ACTGCTCACA AGAAAAAAGG CACG (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made bymCherry gene from Tsien Lab
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pHL662 was a gift from Han Lim (Addgene plasmid # 37636 ; http://n2t.net/addgene:37636 ; RRID:Addgene_37636) -
For your References section:
Rapid and robust signaling in the CsrA cascade via RNA-protein interactions and feedback regulation. Adamson DN, Lim HN. Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 10.1073/pnas.1308476110. Epub 2013 Jul 22. 10.1073/pnas.1308476110 PubMed 23878244
Map uploaded by the depositor.