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Plasmid#162907PurposeGateway attB2r/attB3 entry clone for construction of C-terminal mRuby3 fusion proteinsDepositorInsertmonomeric Ruby3 fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
BS-TRP1-TetOff(7 repeats)-mRuby3-Pho8
Plasmid#207031PurposeTet-Off controlled mRuby3-Pho8 with TRP1 selection. Tetracycline-controlled transactivator (tTA) present on same plasmid.DepositorInsertPho8
UseTagsmRuby3ExpressionYeastMutationPromoterAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFSynW pHluorin-SYT9 IRES mRuby3
Plasmid#195698PurposeLentiviral plasmid encoding SYT9 with an N-terminal pHluorin tag followed by an internal ribosomal entry site followed by mRuby3 under the human synapsin promoterDepositorInsertSyt9 (Syt5 Rat)
UseLentiviralTagspHluorinExpressionMutationPromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mRuby3
Plasmid#112005PurposeFor enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3DepositorHas ServiceAAV5 and AAV9Insertaxon-GCaMP6s-P2A-mRuby3
UseAAVTagsGAP43 palmitoylation domainExpressionMammalianMutationPromoterhSynapsin1Available sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-jGCaMP8m-P2A-mRuby3
Plasmid#172922PurposeFor enriched expression of jGCaMP8m in axons with cytosolic expression of mRuby3DepositorHas ServiceAAV1 and AAV9Insertaxon-jGCaMP8m-P2A-mRuby3
UseAAVTagsGAP43 palmitoylation domain (N terminal on insert…ExpressionMammalianMutationPromoterhSynapsin1Available sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-jGCaMP8f-P2A-mRuby3
Plasmid#172923PurposeFor enriched expression of jGCaMP8f in axons with cytosolic expression of mRuby3DepositorHas ServiceAAV1 and AAV9Insertaxon-jGCaMP8f-P2A-mRuby3
UseAAVTagsGAP43 palmitoylation domain (N terminal on insert…ExpressionMammalianMutationPromoterhSynapsin1Available sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-ChroME2s-ST-P2A-NLS-mRuby3
Plasmid#170177PurposeCation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorInsertChroME2s-ST-P2A-NLS-mRuby3
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3
Plasmid#172921PurposeFor enriched expression of jGCaMP8s in axons with cytosolic expression of mRuby3DepositorHas ServiceAAV1 and AAV9Insertaxon-jGCaMP8s-P2A-mRuby3
UseAAVTagsGAP43 palmitoylation domain and P2A-mRuby3ExpressionMammalianMutationPromoterhSynapsin1Available sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-ChroME2f-ST-P2A-NLS-mRuby3
Plasmid#170175PurposeCation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorInsertChroME2f-ST-P2A-NLS-mRuby3
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKK-BI16-ORF1-3C-mRuby3_ORF2-TEV-mClover3
Plasmid#105802PurposeConcomitant expression of two proteins. One protein expressed with mRuby3, cleavable by 3C; second protein expressed with mClover3, cleavable by TEV protease; tags positions: C-termini.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: 3C-mRuby3; ORF2: TEV-mClover3ExpressionMammalianMutationPromoterAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
C512-E23: attB5r-mRuby3-nostop-attB1r
Plasmid#162945PurposeGateway attB5r/attB1r entry clone for generation of N-terminal mRuby3 fusion proteinsDepositorInsertmonomeric Ruby3 fluorescent protein
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceApril 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX552-DIO-NLS-mRuby3(with gRNA scaffold)
Plasmid#199583PurposeExpresses NLS-mRuby3 in a Cre-dependent mannerDepositorInsertNLS-mRuby3
UseAAV, CRISPR, and Cre/LoxTagsExpressionMammalianMutationPromoterU6 and EF1aAvailable sinceOct. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-FLEX-NEPLDCV-P2A-mRUBY3-WPRE
Plasmid#207664PurposeNeuropeptide degradationDepositorInsertNEP (MME Human)
UseAAV and Cre/LoxTagsmRUBY3ExpressionMutationAddition of signaling peptide (POMC) on the N-ter…PromoterhSynapsinAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-FLEx-axon-GCaMP6s-P2A-mRuby3
Plasmid#112008PurposeFor cre-dependent enriched expression of GCaMP6s in axons with cytosolic expression of mRuby3DepositorInsertaxon-GCaMP6s-P2A-mRuby3
UseAAV and Cre/LoxTagsGAP43 palmitoylation domainExpressionMammalianMutationPromoterhSynapsin1Available sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-jGCamp7b-P2A-GSG-mRuby3
Plasmid#130900Purposebicistronic plasmid for dual-color neuronal calcium and morphology imagingDepositorInsertjGCaMP7b-P2A-mRuby3
UseAAVTagsExpressionMammalianMutationPromoterhSynAvailable sinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-ChroME2s-ST-P2A-H2B-mRuby3
Plasmid#170163PurposeCation channelrhodopsin ChroME2s targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorInsertChroME2s-P2A-H2B-mRuby3
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-ChroME2f-ST-P2A-H2B-mRuby3
Plasmid#171150PurposeCation channelrhodopsin ChroME2f targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorInsertChroME2f-P2A-H2B-mRuby3
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-ChroME-ST-P2A-H2B-mRuby3
Plasmid#108912PurposeCation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorHas ServiceAAV9InsertChroME-ST
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-DIO-NLS-mRuby3-IRES-eGtACR1-ST
Plasmid#109048PurposeAnion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequence in a viral vectorDepositorHas ServiceAAV9InserteGtACR1-ST
UseAAVTagsExpressionMutationPromoterCAGAvailable sinceJuly 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-ChroME-ST-P2A-H2B-mRuby3
Plasmid#170161PurposeCation channelrhodopsin ChroME targeted to the neuronal soma and proximal dendrites and separated from nuclear mRuby3 by P2A in a viral vectorDepositorInsertChroME-P2A-H2B-mRuby3
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only