Addgene

We narrowed to 5,521 results for: #16

Showing: 1521 - 1540 of 5521 results

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pSin wPGK-Cre

Plasmid

#101242

Purpose

low-efficiency Cre used to turn on dio YFP/ PSD95-mCherry/ Teal-gephyrin expression

Depositor

Elly Nedivi

Article

Villa et al Neuron. 2016 Feb 3. pii: S0896-6273(16)0

Insert

Cre recombinase

Use

Lentiviral

Tags

Expression

Mutation

see attached file

Promoter

Available since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pSOMA-NLS

Plasmid

#118937

Purpose

Used to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana

Depositor

Patrick Krysan

Article

Zaman et al Plant J. 2018 Nov 16. doi: 10.1111/tpj.1

Insert

SOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT TO myc hSeparase

Plasmid

#59820

Purpose

Allows the integration of myc hSeparase in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Cell Sci. 2006 Aug 15;119(Pt 16):3325-

Insert

Separase (ESPL1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pLacI-LAP-BUB1

Plasmid

#115905

Purpose

Used to express BUB1-WT fused to LacI for recruitment of BUB1 to LacO spots.

Depositor

Geert Kops

Article

Vleugel et al J Cell Sci. 2015 Aug 15;128(16):2975-82.

Insert

LacI-BUB1 (BUB1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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LRP6 deltaN-pCS2-VSVG

Plasmid

#27281

Depositor

Xi He

Article

Tamai et al Mol Cell. 2004 Jan 16. 13(1):149-56.

Insert

low density lipoprotein receptor-related protein 6 (LRP6 Human)

Use

Xenopus injection

Tags

VSVG

Expression

Mutation

contains amino acid residues 1370–1613 (extracell…

Promoter

Available since

Jan. 26, 2011

Availability

Academic Institutions and Nonprofits only

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AF1521-c002

Plasmid

#173109

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))

Use

Tags

mCerulean

Expression

Bacterial

Mutation

KH-M1-L192

Promoter

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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CAG-SaCas9-WPRE

Plasmid

#89996

Purpose

Expresses high levels of WT SaCas9 for genome editing

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Res. 2017 Jul;22:16-19. doi: 1

Insert

SaCas9 (NEWENTRY Staphylococcus aureus)

Use

Tags

3xHA, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 28, 2017

Availability

Academic Institutions and Nonprofits only

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1.5 BV

Plasmid

#16048

Depositor

Robert Benezra

Article

Tournay et al Mol Cell Biol. 1996 May . 16(5):2418-30.

Insert

Id1 promoter region (Id1 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

-1585 to +88 of promoter

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
LZRS PU.1 WT

Plasmid

#34835

Depositor

Ellen Rothenberg

Article

Anderson et al Immunity. 2002 Feb;16(2):285-96.

Insert

PU.1 (Spi1 Mouse)

Use

Retroviral

Tags

EGFP and IRES

Expression

Mammalian

Mutation

Promoter

LTR

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
GluR2_adRNA(1,20,6)-ADAR2(E488Q)

Plasmid

#124621

Purpose

Expresses adRNA(1,20,6) targeting the RAB7A transcript along with the ADAR2(E488Q)

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

ADAR2(E488Q)

Use

AAV

Tags

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+MT-hToca-1-W518K

Plasmid

#33031

Depositor

Marc Kirschner

Article

Ho et al Cell. 2004 Jul 23;118(2):203-16.

Insert

Toca-1 (FNBP1L Human)

Use

Tags

6xMyc

Expression

Mammalian

Mutation

W518K

Promoter

simian CMV IE94

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-SPORT6-RNF114-RINGmut

Plasmid

#69922

Purpose

Expresses mutant myc-RNF114 in mammalian cells

Depositor

Francesca Capon

Article

Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

Insert

RNF114 (RNF114 Human)

Use

Tags

Expression

Mammalian

Mutation

C29A, C32A

Promoter

CMV

Available since

Feb. 25, 2016

Availability

Academic Institutions and Nonprofits only
RabV CVS-N2c(deltaG)-mCherry-P2A-FlpO

Plasmid

#73471

Purpose

Expresses mCherry and FlpO for monosynaptic circuit mapping and genetic manipulation

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

mCherry-P2A-FlpO

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:(MCS)-S11-DI-GFP1–9

Plasmid

#108260

Purpose

Binary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plants

Depositor

Tzu-Yin Liu

Article

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

UBQ10:sXVE:

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_UEVLD_UEV

Plasmid

#109902

Purpose

Protein expression and purification of UEVLD_UEV

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

UEVLD_UEV (UEVLD Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NR1D1_Hormone-recep

Plasmid

#109960

Purpose

Protein expression and purification of NR1D1_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR1D1_Hormone-recep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pBEST_OR2-OR1-Pr-UTR1_ClpP-T500

Plasmid

#153302

Purpose

Expresses ClpP protease in the cell-free TX-TL system

Depositor

Mikhail Shapiro

Article

Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

Insert

clpP (clpP Escherichia coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_PCMT1_PCMT

Plasmid

#109932

Purpose

Protein expression and purification of PCMT1_PCMT

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PCMT1_PCMT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-Amida

Plasmid

#15362

Depositor

Ronald Conaway, Joan Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

TCF3 (E2A) fusion partner (TFPT Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT/TO_CEP41-HaloTag7_T2A_EGFP

Plasmid

#169327

Purpose

Expression of CEP41-HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CEP41-HaloTag7 (CEP41 Human)

Use

Tags

HaloTag7

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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