Showing: 1461 - 1480 of 2668 results
-
Plasmid#110823PurposePiggyBac compatible plasmid expressing dCas9DepositorInsertdCas9
UseTagsExpressionMammalianMutationCas9 mutations to make dCas9=D10A+D839A+H840A+N86…PromoterCAGGSAvailabilityAcademic Institutions and Nonprofits only -
pLenti-2X-P2A-Puro
Plasmid#110840PurposeLentiviral vector for constitutive expression of 2X in mammalian cells (codon optimized)DepositorInsertBE3RA-2X
UseLentiviralTagsExpressionMutationD10A and 2 tandem NLS sequences in the XTEN linkerPromoterEF1sAvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLS-P2A-Puro
Plasmid#110841PurposeLentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGExpressionMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLS-PGK-Puro
Plasmid#110844PurposeLentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGExpressionMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailabilityAcademic Institutions and Nonprofits only -
pLenti-TRE3G-FNLS-PGK-Puro
Plasmid#110847PurposeLentiviral vector for dox-inducible expression of FNLS in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGExpressionMutationD10A and NLS sequence at the N-terminusPromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3-P2A-GFP-PGK-Puro
Plasmid#110867PurposeLentiviral vector for constitutive expression of BE3-P2A-GFP (not codon optimized)DepositorInsertBE3
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE3RA-P2A-GFP-PGK-Puro
Plasmid#110868PurposeLentiviral vector for constitutive expression of BE3RA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertBE3RA
UseLentiviralTagsExpressionMutationD10APromoterEF1sAvailabilityAcademic Institutions and Nonprofits only -
pET-dSpCas9-HF1-VP64-6xHis
Plasmid#92118PurposeExpression of dead/inactive increased fidelity SpCas9-HF1-VP64-6xHis in bacterial cellsDepositorInsertdead/inactive SpCas9-HF1-NLS-3xFLAG-VP64
UseCRISPRTags3xFLAG, 6xHis, NLS, and VP64ExpressionBacterialMutationD10A, N497A, R661A, Q695A, H840A, Q926APromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry
Plasmid#122132PurposeExpress a dCas9, 10X GCN4, and mCherry cassette from the TRE3G promoterDepositorInsertdCas9-10X GCN4-P2A-mCherry
UseCRISPR and LentiviralTagsExpressionMammalianMutationdCas9 D10A & H840APromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
LLP457 pGK-dCas9-Suntag-BFP
Plasmid#100957PurposeBPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tagDepositorInsertdCas9, SunTag array (10xGCN4)
UseCRISPRTags3xHA and BFPExpressionMammalianMutationD10A, H840A for dCas9PromoterAvailabilityAcademic Institutions and Nonprofits only -
pIA33
Plasmid#120803PurposeE. coli-C. difficile shuttle vector for CRISPR interference (CRISPRi) in C. difficile; sgRNA targets rfp; Pxyl::dCas9-opt Pgdh::sgRNA-rfpDepositorInsertsdeactivated nuclease dCas9, codon-optimized for C. difficile
single guide RNA targeting red fluorescent protein
UseCRISPRTagsExpressionBacterialMutationBase-pairing region targets red fluorescent prote…PromoterPgdh and PxylAvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE4RA-P2A-Puro
Plasmid#112672PurposeLentivirus for constitutive expression of BE4RA in mammalian cells (codon optimized)DepositorInsertBE4RA
UseLentiviralTagsFLAGExpressionMutationNLS sequence at the N-terminus and D10APromoterEF1sAvailabilityAcademic Institutions and Nonprofits only -
pLenti-BE4GamRA-P2A-Puro
Plasmid#112673PurposeLentivirus for constitutive expression of BE4GamRA in mammalian cells (codon optimized)DepositorInsertBE4GamRA
UseLentiviralTagsFLAGExpressionMutationNLS sequence at the N-terminus and D10APromoterEF1sAvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA1-BE3
Plasmid#113418PurposeExpresses hA1-BE3 in mammalian cellsDepositorInserthA1-BE3 (APOBEC1 Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCMV-mAID-BE3
Plasmid#113420PurposeExpresses mAID-BE3 in mammalian cellsDepositorInsertmAID-BE3 (Aicda Mouse, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-eBE-Y130F
Plasmid#113423PurposeExpresses hA3A-eBE-Y130F in mammalian cellsDepositorInserthA3A-eBE-Y130F (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationhAPOBEC3A_Y130FPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCMV-hA3A-eBE-Y132D
Plasmid#113429PurposeExpresses hA3A-eBE-Y132D in mammalian cellsDepositorInserthA3A-eBE-Y132D (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
UseCRISPRTagsExpressionMammalianMutationhAPOBEC3A_Y132DPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Cas9-T2A-GFP-3xP3-RFP
Plasmid#127560PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Cas9-T2A-GFP-SV40 and 3xP3-RFP visible eye marker.DepositorInsertCas9-T2A-GFP
UseCRISPRTagsExpressionInsectMutationEGFP-D102G see depositor comments belowPromoterAvailabilityAcademic Institutions and Nonprofits only -
pLY152
Plasmid#130950Purposedcas9 generator and reporter circuit (PpspA-2G6 with sfgfp::ASV)DepositorInsertsdcas9
tetR
sfgfp
UseSynthetic BiologyTagsASV tagExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterPpspA-2G6 and PtetAvailabilityAcademic Institutions and Nonprofits only -
pRS0045
Plasmid#131124PurposeMammalian Target-ACE expression vectorDepositorInsertTadA-linker(32aa)-TadA*(7.10)-linker(32aa)-nCas9-PmCDA1-UGI
UseCRISPRTagsPmCDA1, TadA, and UGIExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219V …PromoterCMV promoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 1461 - 1480 of 2668 results