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Addgene

pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry
(Plasmid #122132)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122132 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHR
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    mCherry

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9-10X GCN4-P2A-mCherry
  • Mutation
    dCas9 D10A & H840A
  • Promoter TRE3G

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHR-TRE3G-dCas9-10X GCN4-P2A-mCherry was a gift from Stanley Qi (Addgene plasmid # 122132 ; http://n2t.net/addgene:122132 ; RRID:Addgene_122132)

  • For your References section:

    CRISPR Activation Screens Systematically Identify Factors that Drive Neuronal Fate and Reprogramming. Liu Y, Yu C, Daley TP, Wang F, Cao WS, Bhate S, Lin X, Still C 2nd, Liu H, Zhao D, Wang H, Xie XS, Ding S, Wong WH, Wernig M, Qi LS. Cell Stem Cell. 2018 Sep 28. pii: S1934-5909(18)30440-5. doi: 10.1016/j.stem.2018.09.003. 10.1016/j.stem.2018.09.003 PubMed 30318302
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