Addgene

We narrowed to 1,935 results for: eda

Showing: 1361 - 1380 of 1935 results

Results per page:

Sort results by:

pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP

Plasmid

#126698

Purpose

to express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

McCauley et al Stem Cell Reports. 2018 May 8;10(5):1579

Insert

sgRNA targeting human SCGB3A2 locus

Use

Tags

Expression

Mammalian

Mutation

Promoter

Human U6

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
proISG15 (2-165)

Plasmid

#110762

Purpose

Bacterial expression of proISG15

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

ISG15 (ISG15 Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCD1C

Plasmid

#102954

Purpose

gene cloning of human CD1C

Depositor

Gennaro De Libero, Lucia Mori

Article

Lepore et al J Exp Med. 2014 Jun 30;211(7):1363-77. d

Insert

CD1C cDNA (CD1C Human)

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-actin prom-dsRed-adra2a.1227.shRNA

Plasmid

#67880

Purpose

expresses dsRed and shRNA to knock down adra2a receptors

Depositor

William Wisden

Article

Zhang et al Nat Neurosci. 2015 Apr;18(4):553-61. doi

Insert

dsRed and shRNA to knock down the mouse adra2 receptor

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

chicken beta actin

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only
pMSCV-IRES-GFP/CALR ins5

Plasmid

#214701

Purpose

Mammalian expression of human CALR ins5

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR ins5 (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-IRES-GFP/CALR del52

Plasmid

#214700

Purpose

Mammalian expression of human CALR del52

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-IRES-GFP/CALR wt

Plasmid

#214699

Purpose

Mammalian expression of human CALR wt

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR wt (CALR Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

MSCV

Available since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEMHE-mScarlet-hCenpC

Plasmid

#171490

Purpose

T7 promotor drives in vitro transcription of mScarlet-tagged human hCenpC mRNA

Depositor

Melina Schuh

Article

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

CenpC (CENPC Human)

Use

Pgehme

Tags

mScarlet

Expression

Mutation

Promoter

T7

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-GRET

Plasmid

#158222

Purpose

Control lentiviral plasmid encoding GFP-Nluc BRET reporter from Schaub et al., Cancer Research, 2015.

Depositor

Charles P. Lai

Article

Wu et al Adv Sci (Weinh). 2020 Aug 16;7(19):20014

Insert

GFP-Nluc

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pg-proPHD2-PHD2-EGFP

Plasmid

#87210

Purpose

PHD2-EGFP expressed under the control of the PHD2 promoter

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

PHD2 (EGLN1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

H313N

Promoter

PHD2

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB CAG SMARCA4-IRES-EGFP

Plasmid

#153948

Purpose

piggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFP

Depositor

Luca Tiberi

Article

Ballabio et al Nat Commun. 2020 Jan 29;11(1):583. doi:

Insert

SMARCA4 (SMARCA4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pet21a-aGFPnb-minimizer-cys-linker-YbbR-(PAS)5-H6

Plasmid

#192789

Purpose

aGFP nanobody minimizer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

antiGFP nanobody minimizer

Use

Tags

H6 and ybbR

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Met1 diUb (1-152)

Plasmid

#110766

Purpose

Bacterial expression of Met1 diUb

Depositor

David Komander

Article

Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

Insert

UBB (UBB Human)

Use

Tags

N-His6-3C

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
3I91

Plasmid

#62514

Purpose

Bacterial expression plasmid for use in structural determination.

Depositor

Cheryl Arrowsmith

Article

Kaustov et al J Biol Chem. 2011 Jan 7;286(1):521-9. do

Insert

CBX8 (CBX8 Human)

Use

Tags

6xHis and TEV cleavage

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pLKO_HOXB13_#2

Plasmid

#70094

Purpose

Expression of shRNA to human HOXB13, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pLKO_HOXB13_#1

Plasmid

#70093

Purpose

Expression of shRNA to human HOXB13, puromycin selection

Depositor

William Hahn

Article

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

HOXB13 (HOXB13 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pKI-cjFOXP2-hum

Plasmid

#129757

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

FOXP2 (LOC100411307 C. jacchus (common marmost))

Use

Marmoset targeting

Tags

Expression

Mutation

T301N/N323S

Promoter

Available since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

Plasmid

#192788

Purpose

aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

Depositor

Jacob Piehler

Article

Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

Insert

antiGFP nanobody enhancer

Use

Tags

H6 and ybbR

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-PRMT6(V86K/D88A)

Plasmid

#164698

Purpose

Expression of PRMT6(V86K/D88A) with an N-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Article

Eram et al ACS Chem Biol. 2016 Mar 18;11(3):772-781

Insert

PRMT6 (PRMT6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

V86K, D88A

Promoter

CMV

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-MHL: nsp16_SARS-CoV-2|nsp10_SARS-CoV-2

Plasmid

#167849

Purpose

Bacterial expression of the SARS-CoV-2 proteins nsp10 and nsp16 from a bicistronic mRNA

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10, nsp16

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 22, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge