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Showing: 101 - 120 of 177 results

  13. GFP-LiMETER-v2

    Plasmid
    #113934
    Purpose
    Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP
    Depositor
    Yubin Zhou
    Article
    He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi
    Insert
    LOV2 and Stim1 polybasic domain (PB)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    113934-plasmid-map-sequence-id-225307

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  16. pROS13_IDH2 #7

    Plasmid
    #166102
    Purpose
    This plasmid express a sgRNA that targets the IDH2 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH2-135 by homologous recombination.
    Depositor
    Matthias Heinemann
    Article
    Chen et al bioRxiv
    Insert
    IDH2 sgRNA #7 (IDH2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    166102-plasmid-map-sequence-id-330316

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  17. pROS13_IDH1 #4

    Plasmid
    #166101
    Purpose
    This plasmid express a sgRNA that targets the IDH1 gene. This allows Cas9 to make a double-strand break and facilitate integration of LOV2 at position IDH1-67 by homologous recombination.
    Depositor
    Matthias Heinemann
    Article
    Chen et al bioRxiv
    Insert
    IDH1 sgRNA #4 (IDH1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    April 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    166101-plasmid-map-sequence-id-330298

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  19. pTriEx-mVenus-PA-Rac1

    Plasmid
    #22007
    Purpose
    Expression of photoactivatable Rac1
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1 (RAC1 Avena sativa (oat), Human)
    Use
    Tags
    6X His and mVenus
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546), Rac1 starts at I4 and contain…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
Showing: 101 - 120 of 177 results