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Addgene

mCherry-PI(WT)-Vav2(DPZ)
(Plasmid #86974)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 86974 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pmCherry
  • Backbone size w/o insert (bp) 3969
  • Total vector size (bp) 7734
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    LOV2
  • Species
    Synthetic
  • Insert Size (bp)
    447
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    VAV2 DH/PH/C1
  • Alt name
    VAV2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1200
  • Entrez Gene
    Vav2 (a.k.a. 2810040F13Rik, AI847175, Vav-2)
  • Promoter CMV

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-PI(WT)-Vav2(DPZ) was a gift from Klaus Hahn (Addgene plasmid # 86974 ; http://n2t.net/addgene:86974 ; RRID:Addgene_86974)
  • For your References section:

    Engineering extrinsic disorder to control protein activity in living cells. Dagliyan O, Tarnawski M, Chu PH, Shirvanyants D, Schlichting I, Dokholyan NV, Hahn KM. Science. 2016 Dec 16;354(6318):1441-1444. 10.1126/science.aah3404 PubMed 27980211