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We narrowed to 11 results for: LOV2

Showing: 1 - 11 of 11 results

  2. Hot Plasmids - March 2019 - Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins

    Type
    Blog Post
    ...accomplished this by inserting a photo-sensitive LOV2 domain from A. sativa into a loop region of the ...addition of blue light, the terminal helices of the LOV2 domain unfold, disrupting the protein conformation...deposited for the photoswitchable AsLOV2. Find the cPYP and AsLOV2 protein binder plasmids here! Reis...binder tools for photoswitchable proteins cPYP and AsLOV2 Article contributed by Angela Abitua  Listen to...

  4. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    ...AcrIIA4, to a photo-sensitive LOV2 domain from A. sativa (Bubeck et al., 2018). LOV2 has a C-terminal helix ...Light-Dependent Anti-CRISPR Bubeck et al., 2018 CASANOVA LOV2 from A. Sativa AcrIIA4 anti-CRISPR protein, co-expressed...

  6. Optogenetics Guide

    Type
    Guide
    ...dimers; Bacterial SsrA peptide is embedded in the LOV2 domain (iLID plasmid), while its natural binding...activation induces a conformational change in the LOV2 domain which allows SsrA to bind with SspB LAD Light-activated...light-inducible nuclear localization signal based on the LOV2 domain of Avena sativa phototropin 1 LOVETRAP reversibly...in the dark. attaching only one member of the Zdk/LOV2 pair to the target protein, and the other to the...Peptide toxin for blocking K + (Kv) channels fused to LOV2 photoswitch. Illumination causes the photoswitch...

  7. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ... with the light-sensitive LOV2 domain of Avena Sativa phototropin 1 (AsLOV2). In the dark, this peptide...peptide is caged by the asLOV2 domain and has reduced affinity for its binding target, SspB. In the light,...light, the Jα helix of the asLOV2 domain unfolds allowing it to bind to SspB. This original Light Inducible...

  8. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

    Type
    Blog Post
    ...OptoNBs have two key components: an AsLOV2 domain and a nanobody.  AsLOV2 is a light-oxygen-voltage sensing...important to insert AsLOV2 at a spot in the nanobody that preserves the activity of both AsLOV2 and the nanobody... the oat plant (Avena sativa). In darkness, the AsLOV2 domain folds back on itself in a closed conformation...interact? When fused together to form an OptoNB, the AsLOV2 domain acts like an on/off switch for the nanobody...Generating OptoNBs The opening and closing of the AsLOV2 domain allosterically alters the shape and binding...proof-of-principle the Toettcher Lab monitored how AsLOV2 insertion into mCherry or GFP nanobodies affected...loops of nanobodies seemed like a good place to put AsLOV2 because here it was less likely to destroy the ...

  9. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    ...had shown magnetic responses: EGFP, mScarlet, and AsLOV2. After several rounds of semi-random mutagenesis...she deprioritize the project, Maria’s screens of AsLOV2 variants showed some significant gains! After a...mutagenesis, they eventually found a version of AsLOV2 with five mutations (C450P, L496V, Q513K, G528K...Fluorescence intensity and ΔF/F of MagLOV, a variant of AsLOV2 with mutations C450P, L496V, Q513K, G528K, D540M...optogenetic tools. And, since MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic...

  10. CRISPR Guide

    Type
    Collection
    ...anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled Acr activity...

  11. CRISPR Guide

    Type
    Guide
    ...anti-CRISPR protein AcrIIA4 with the photosensitive LOV2 domain . Several labs have also controlled Acr activity...
Showing: 1 - 11 of 11 results