...Addgene). It consists of two domains, LOV2 and Zdk1, where Zdk1 binds LOV2 with high affinity in the dark but...can be switched off with blue light which causes LOV2 and Zdk1 to dissociate due to conformational changes...

...accomplished this by inserting a photo-sensitive LOV2 domain from A. sativa into a loop region of the ...addition of blue light, the terminal helices of the LOV2 domain unfold, disrupting the protein conformation...deposited for the photoswitchable AsLOV2. Find the cPYP and AsLOV2 protein binder plasmids here! Reis...binder tools for photoswitchable proteins cPYP and AsLOV2 Article contributed by Angela Abitua  Listen to...

... Streptococcus pyogenes Cas9 inhibitor, with the LOV2 photosensor from Avena sativa, light-mediated genome...

...AcrIIA4, to a photo-sensitive LOV2 domain from A. sativa (Bubeck et al., 2018). LOV2 has a C-terminal helix ...Light-Dependent Anti-CRISPR Bubeck et al., 2018 CASANOVA LOV2 from A. Sativa AcrIIA4 anti-CRISPR protein, co-expressed...

...For example, fusing AcrA4II to the photo-sensitive LOV2 domain allows anti-CRISPR activity to be controlled...

... with the light-sensitive LOV2 domain of Avena Sativa phototropin 1 (AsLOV2). In the dark, this peptide...peptide is caged by the asLOV2 domain and has reduced affinity for its binding target, SspB. In the light,...light, the Jα helix of the asLOV2 domain unfolds allowing it to bind to SspB. This original Light Inducible...

...OptoNBs have two key components: an AsLOV2 domain and a nanobody.  AsLOV2 is a light-oxygen-voltage sensing...important to insert AsLOV2 at a spot in the nanobody that preserves the activity of both AsLOV2 and the nanobody... the oat plant (Avena sativa). In darkness, the AsLOV2 domain folds back on itself in a closed conformation...interact? When fused together to form an OptoNB, the AsLOV2 domain acts like an on/off switch for the nanobody...Generating OptoNBs The opening and closing of the AsLOV2 domain allosterically alters the shape and binding...proof-of-principle the Toettcher Lab monitored how AsLOV2 insertion into mCherry or GFP nanobodies affected...loops of nanobodies seemed like a good place to put AsLOV2 because here it was less likely to destroy the ...

...had shown magnetic responses: EGFP, mScarlet, and AsLOV2. After several rounds of semi-random mutagenesis...she deprioritize the project, Maria’s screens of AsLOV2 variants showed some significant gains! After a...mutagenesis, they eventually found a version of AsLOV2 with five mutations (C450P, L496V, Q513K, G528K...Fluorescence intensity and ΔF/F of MagLOV, a variant of AsLOV2 with mutations C450P, L496V, Q513K, G528K, D540M...optogenetic tools. And, since MagLOV’s parent protein AsLOV2 has already been adapted into a variety of optogenetic...