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Plasmid#178276PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsHSV.Ollas.S and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.HSV.Ollas_mCherry-NLS
Plasmid#178259PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsHA.HSV.Ollas and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO_HA.HSV.NWS_mCherry-NLS
Plasmid#178258PurposeNuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 trRNA cassette for sgRNA cloning. Enables spatial detection of CRISPR screens.DepositorInsertnPC Tagged mCherry-NLS
UseLentiviralTagsHA.HSV.NWS and Nuclear Localization SignalExpressionMutationPromotereF1aAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
AOI-WT-Cas9-sg-mouse Suz12-F2-GFP
Plasmid#91882PurposeExpresses 3xFLAG-Cas9 and a gRNA targeting mouse Suz12DepositorInsertgRNA targeting Suz12 (Suz12 Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC35F5
Plasmid#132085PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC35F5 (SLC35F5 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO.TRC1.shmNsd1.2, puro
Plasmid#114447PurposeLentiviral vector for expression of shRNA sequence targeting mouse Nsd1DepositorInsertshNsd1.2 (Nsd1 Mouse)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLKO.TRC1.shmNsd1.1, puro
Plasmid#114446PurposeLentiviral vector for expression of shRNA sequence targeting mouse Nsd1DepositorInsertshNsd1.1 (Nsd1 Mouse)
UseLentiviral and RNAiTagsExpressionMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pN1 VAMP2 R125TAG
Plasmid#69877Purposeexpress in mammalian cells for unnatural amino acid incorporation into VAMP2DepositorInsertVAMP2 (Vamp2 Rat)
UseSynthetic BiologyTagsExpressionMammalianMutationAmber and Ochre stop codons in the former linker …PromoterAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 S248A
Plasmid#182047Purposeexpression of Sox2 mutantsDepositorInsertSox2 (Sox2 Mouse)
UseTagsFLAGExpressionMammalianMutationS248APromoterCAGAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 S248A/T258A
Plasmid#182049Purposeexpression of Sox2 mutantsDepositorInsertSox2 (Sox2 Mouse)
UseTagsFLAGExpressionMammalianMutationS248A/T258APromoterCAGAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FLAG-IRES-blasticidin-Sox2 T258A
Plasmid#182048Purposeexpression of Sox2 mutantsDepositorInsertSox2 (Sox2 Mouse)
UseTagsFLAGExpressionMammalianMutationT258APromoterCAGAvailable sinceApril 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCR3-Flag-AuroraB KD (K106R)
Plasmid#108488Purposeexpression of Flag-AuroraB kinase death (K106R)DepositorInsertAuroraB (AURKB Human)
UseTagsFlagExpressionMammalianMutationKinase death K106APromoterCMVAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-YOD1-MutS2-C160S
Plasmid#85668PurposeExpression of human YOD1 I292Q V295Q C160S mutant with N-terminal GFP tagDepositorInsertYOD1 (YOD1 Human)
UseTagsGFPExpressionMammalianMutationC160S and I292Q and V295QPromoterCMVAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pShuttle-EGFP-CLASP2 340-1362 9xS/A
Plasmid#24373DepositorInsertCLASP2 (340-1362) 9xS/A (CLASP2 Human)
UseTagsEGFPExpressionMammalianMutationNonphosphorylatable CLASP2 deletion mutant. M…PromoterAvailable sinceJune 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC2A7
Plasmid#132066PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC2A7 (SLC2A7 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLKO.1_sh_ex13
Plasmid#35165DepositorInsertFoxp1 (Foxp1 Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC35A3
Plasmid#132135PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC35A3 (SLC35A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
CMV-218_HNF4A2
Plasmid#31090DepositorInsertHNF4A (HNF4A Human)
UseFlp/frtTagsmycExpressionMammalianMutationsplice variant 2 of HNF4A, deletion of CMV promo…PromoterAvailable sinceOct. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc-oDam_f_GFPoNLS
Plasmid#85818PurposeiDamID plasmid. To express transiently the c-Myc-tagged and optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc oDam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A. Codon optimization compatible to work in M…PromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT5T_hp53_ST_CR2
Plasmid#34923DepositorInsertp53 DNA-binding (Res 94-358, deletion 293-321) (TP53 Human)
UseTagsExpressionBacterialMutationC135V, C141V, W146Y, C182S, V203A, R209P, C229Y, …PromoterT7Available sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only