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Plasmid#198863PurposePlasmid encoding for 2 gRNAs targeting the human TERT geneDepositorInsertTERT sgRNA (TERT Human)
UseTagsExpressionMammalianMutationPromoteru6Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSCALPS-Flag-Mili-EGFP
Plasmid#212576PurposeLentiviral expression of Flag-tagged mouse Mili in mammalian cellsDepositorInsertMili (Piwil2 Mouse)
UseLentiviralTags5x Flag and SNAP tagExpressionMammalianMutationPromoterSFFVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
BII-gR-BtW-eSpCas9
Plasmid#133358PurposePiggybac vector for CRISPR/SpCas9 applications (nuclease, base editing, or epigenetic modifications). Provides gRNA and EspCas9 expressionDepositorInserteSpCas9
UseTagsFlag-tagged eSpCas9ExpressionMammalianMutationeSpCas9 mutationsPromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCarCBADE_ProB(I69E)A
Plasmid#117756PurposeProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backboneDepositorInsertGlutamate kinase (ProA), mutant pyrroline-5-carboxylate synthase (ProBI69E) from E. coli and codon optimised CarCBADE operon
UseTagsExpressionBacterialMutationPromoterPLac(UV5)Available sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pLX-Z4 (GB3375)
Plasmid#160648PurposepLX series: RK2-based T-DNA binary vector for plant transformation (alpha1)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAVA-LGF2-Gal11p
Plasmid#127481PurposePositive control for the AVA-seq system. LGF2 (lambda CI associated) and Gal11p (RNAp associated) interactionDepositorInsertLGF2-Gal11p (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAVA-Gal11p-LGF2
Plasmid#127480PurposePositive control for the AVA-seq system. Gal11p (lambda CI associated) and LGF2 (RNAp associated) interactionDepositorInsertGal11p-LGF2 (GAL11 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-ASCL2
Plasmid#133375PurposePiggybac vector for constitutive expressionDepositorInsertASCL2 (ASCL2 Human)
UseTagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIC-His-RGS4
Plasmid#204847PurposeExpress His tagged-RGS4 in bacterial cells for protein purificationDepositorInsertRGS4 (RGS4 Human)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRK-FLAG-TRAF4
Plasmid#58316PurposeFLAG-tagged mouse TRAF4DepositorInsertTNF receptor associated factor 4 (Traf4 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRK-HA-TRAF5
Plasmid#58315PurposeHA-tagged mouse TRAF5DepositorInsertTNF receptor associated factor 5 (Traf5 Mouse)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceJuly 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-myc
Plasmid#109422PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and mycExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-TRE-DIO-mRuby2
Plasmid#99117PurposeAn AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein mRuby2DepositorInsertDIO-mRuby2
UseAAVTagsExpressionMammalianMutationPromoterTREAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCA24N-ligase-cTF
Plasmid#87743PurposeIPTG-inducible expression of ligase-cTF fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and cTF (chimeric transcription factor base…ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
WT C-His Ana GvpC in pET28a
Plasmid#85732PurposeExpresses His-tagged GvpC from Anabaena flos-aquae in E.coliDepositorInsertGas vesicle protein C (his-tagged)
UseTagsHis-tagExpressionBacterialMutationAdded an extra glycine after the methionine at th…PromoterT7Available sinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
BII-ChBtW-IGF2
Plasmid#133374PurposePiggybac vector for constitutive expressionDepositorInsertIGF2 (IGF2 Human)
UseTagsFlagExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
evoCas9
Plasmid#138558PurposeExpresses human codon-optimized evolved SpCas9 and blasticidin resistance: EFS promoter-evoCas9-NLS-FLAG-P2A-BSDDepositorInsertevoCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationM495V, Y515N, K526E, R661QPromoterEFSAvailable sinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)
Plasmid#99144PurposeInducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139ADepositorInsertUPF1 (UPF1 Human)
UseLentiviralTagsFlagExpressionMammalianMutationFirst 127 codons are optimized to reduce GC conte…PromoterpTightAvailable sinceNov. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG/hArf4(WT)-mCherry
Plasmid#79406PurposeExpresses C-terminally mCherry-tagged Arf4(WT) in mammalian cellsDepositorInsertArf4 (ARF4 Human)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceApril 12, 2017AvailabilityAcademic Institutions and Nonprofits only