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Plasmid#61762PurposeDual Color betaGlobin reporter to study splicing kinetics in vivoDepositorInsertb-Globin
UseTags24 X MS2 hairpin cassette in 3'UTR of b-Glob…ExpressionMammalianMutationPromoterDox inducible Tet-ON promoterAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
MI-Luciferase-IRES-mCherry
Plasmid#75020PurposeEncodes the expression of Luciferase and mCherryDepositorInsertsfirefly Luciferase
mCherry
UseLuciferase and RetroviralTagsExpressionMutationPromoterAvailable sinceJune 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
Plasmid#222992PurposeMammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNADepositorInsertHuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
UseCRISPRTagsPuroExpressionMammalianMutationPromoterpCAGAvailable sinceJuly 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
miReporter-PGK
Plasmid#82477PurposemicroRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.DepositorInsertH2B-mCherry and H2B-Citrine. HygroR
UseTagsExpressionMutationPromoterAvailable sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
HBV 1.3-mer X-null replicon
Plasmid#65461Purposecontaining 1.3 units of the X-null HBV genome (subtype ayw)DepositorInsert1.3 units of the X-null HBV genome
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
mApple-TfR-RUSH
Plasmid#166906PurposeRUSH cargo: Str-KDEL_IRES_Transferrin Receptor (TfR)-SBP-mAppleDepositorInsertmApple
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJYS1Ptac
Plasmid#85545PurposeConstitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoterDepositorInsertsCpf1
recT
UseCRISPR; Shuttle vector corynebacterium glutamicum…TagsExpressionBacterialMutationPromotertac and unknownAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-rtTA-IRES-eGFP-pGK-NeoR
Plasmid#204497PurposeExpresses rtTA and Neomycin resistance genes in mammalian cellsDepositorInsertrtTA
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K(AAG)20-P2A-RFP
Plasmid#105689PurposeDouble fluorescent stalling reporter K(AAG)20DepositorInsertsP2A-3xFLAG-VHPbeta
K(AAG)20
P2A-mCherry
UseTagsExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pME-mTagBFP-CAAX
Plasmid#75149PurposeMembrane targeted TagBFP (blue) fluorophore for use with 5’ promoters.DepositorInsertmTagBFP-CAAX
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo FlucSM EGFP
Plasmid#90171PurposeEGFP-tagged constructs to monitor the aggregation state of the sensors and the ability of cells to solubilize or degrade the aggregated proteins. FLuc contains single mutationDepositorInsertFLuc EGFP
UseTagsExpressionMammalianMutationR188Q in FLucPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE-VEGFA EF1a-rtTA3
Plasmid#195881PurposeLentiviral construct of doxycycline inducible VEGFADepositorInsertVEGFA (Vegfa Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterTRE3GSAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p221z-CAS9p-TagRFP-t35s
Plasmid#118386PurposeEntry clone containing CAS9p-TagRFP and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertCAS9p-TagRFP
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTF-lacZ
Plasmid#153036PurposeFor CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.DepositorInsertCRISPR array
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYC-N
Plasmid#207113PurposeHDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locusDepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFCcGi
Plasmid#59324PurposeInducible expression of GFP and constitutive expression of mCherryDepositorInsertsmCherry
EGFP
UseTagsExpressionBacterialMutationPromoterPbad and rpsMAvailable sinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby
Plasmid#71761PurposemGFP and Synaptophysin-mRuby expression from Flp-expressing neuronsDepositorInsertsmGFP
Synaptophysin
UseAAVTagsPalmitoylation signal and mRubyExpressionMutationPromoterAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2R3z-BASI-ccdB-BSAI
Plasmid#118389PurposeEntry clone containing ccdB negative selection marker. Used to clone any DNA fragments of interest; goldengate and gibson assembly cloning compatible. For use in plants and compatible with the MultiSite Gateway systemDepositorInsertccdB
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
p5E-smyhc1
Plasmid#126748PurposeZebrafish slow myosin heavy chain promoter. Clone 667DepositorInsertsmyhc1 (smyhc1 Zebrafish)
UseMultisite gateway entry cloneTagsExpressionMutationPromoterAvailable sinceJune 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG_smFP FLAG
Plasmid#59756Purposeencodes multiple copies of an epitope tag FLAGDepositorInsertsmFP _FLAG
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only