Efficient tagging of endogenous proteins in human cell lines for structural studies by single-particle cryo-EM.
Choi W, Wu H, Yserentant K, Huang B, Cheng Y
Proc Natl Acad Sci U S A. 2023 Aug;120(31):e2302471120. doi: 10.1073/pnas.2302471120. Epub 2023 Jul 24.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
207113 | pYC-N | HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus |
207114 | pYC-C | HDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus |