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Efficient tagging of endogenous proteins in human cell lines for structural studies by single-particle cryo-EM.

Choi W, Wu H, Yserentant K, Huang B, Cheng Y
Proc Natl Acad Sci U S A. 2023 Aug;120(31):e2302471120. doi: 10.1073/pnas.2302471120. Epub 2023 Jul 24. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
207113pYC-NHDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus
207114pYC-CHDR donor template with Puromycin resistance and mNeonGreen marker flanked by two multiple cloning sites (MCS) for insertion of left and right homology arms (L- and R-arms) for the targeted locus

Antibodies from Article