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Plasmid#119075PurposeZebrafish ubiquitin promoter driving Com-KRAB with Cerulean reporterDepositorInsertubb:Com-KRAB-2a-Cerulean
UseTagsExpressionBacterialMutationPromoterZebrafish ubiquitin promoterAvailable sinceFeb. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-com-Ds
Plasmid#119077PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x com stem loopsDepositorInsertU6a:2xCom-sgRNA
UseTagsExpressionBacterialMutationPromoterZebrafish U6a promoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pVC-Ds-DrU6a:sgRNA-MS2-Ds
Plasmid#119076PurposeTo clone sgRNA spacer sequence for microinjections. This sgRNA tracrRNA contains 2x MS2 stem loopsDepositorInsertU6a:2xMS2-sgRNA
UseTagsExpressionBacterialMutationPromoterZebrafish U6a promoterAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-myc-PDGFRbeta
Plasmid#136455PurposeMammalian expression plasmid for c-myc-tagged PDGFRBDepositorInsertPDGFRbeta (PDGFRB Human)
UseTagsExtracellular c-myc epitope tag and Signal peptid…ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
CIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q
Plasmid#132545PurposeExpresses CIRTS-8 (bdefensin 3-TBP6.7-hADAR(299-701)E488Q) in mammalian cellsDepositorInsertCIRTS-8: bdefensin 3-TBP6.7-hADAR(299-701)E488Q-NLS
UseTagsFLAG tagExpressionMammalianMutationPromotercmv d0Available sinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1alpha-EPO-218-PDGFR
Plasmid#139057PurposeA lentivirus vector to integrate a surface membrane anchored human EPO geneDepositorInserterythropoietin(EPO) (EPO Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
3xFlag-HaloTag-EZH2 HDR - pDY053
Plasmid#171108PurposeHomology directed repair plasmid for generating HaloTag-EZH2 cellsDepositorInsertEZH2 (EZH2 Human)
UseTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAX-EGFP-CIP4
Plasmid#178363PurposeExpresses CIP4 c-terminal labeled with EGFP in mammalian cellsDepositorInsertCdc42 Interacting Protein 4 (TRIP10 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV-PGKpuro2ABFP
Plasmid#122372PurposeExpresses puro-2A-BFP in mammalian cellsDepositorInsertPuro-2A-BFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETduet-1:MCS1(HemeOxygenase)::MCS2(ReBphP-PCM)
Plasmid#156463PurposeExpress Heme Oxygenase (Nostocaceae) and ReBphP-PCMDepositorInsertsHeme Oxygenase
ReBphP-PCM
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceOct. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgL-FLAG (TB40/E)
Plasmid#136449PurposeMammalian expression plasmid for HCMV glycoprotein L (TB40/E strain) with FLAG tagDepositorInsertglycoprotein L (UL115 Human betaherpesvirus 5)
UseTagsShort SGGSG linker and FLAG tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV.PGK.mLmx1a
Plasmid#33013DepositorInsertLIM homeobox transcription factor 1 alpha (Lmx1a Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
SRSF8_RRM_pGEX
Plasmid#135119PurposeRecombinant expression of SRSF8 with dual-affinity tagDepositorInsertSRSF8 (SRSF8 Human)
UseTagsGST-SBPExpressionBacterialMutationPromotertacAvailable sinceDec. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgO-8his (Merlin)
Plasmid#136454PurposeMammalian expression plasmid for HCMV glycoprotein O (Merlin strain) with an 8his tagDepositorInsertglycoprotein O (UL74 Human betaherpesvirus 5)
UseTagsLinker and 8his tag (GGSGHHHHHHHH, includes BamHI…ExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgH-8his (Merlin)
Plasmid#136447PurposeMammalian expression plasmid for HCMV glycoprotein H (Merlin strain) extracellular domain with 8his purification tagDepositorInsertglycoprotein H (UL75 Human betaherpesvirus 5)
UseTagsShort GGSG linker and 8his tagExpressionMammalianMutationExtracellular domain (a.a. 1-716). Codon-optimize…PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EnhancedSynapsin-DIO-TVA950-EYFP-WPRE
Plasmid#120269PurposeAllows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent proteinDepositorInserteSyn-DIO-TVA950:YFP
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
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M_NLS 67kDa
Plasmid#201351PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40A5-EGFP-6PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
L_NLS 67kDa
Plasmid#201361PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40A4-EGFP-6PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
H_NLS 67kDa
Plasmid#201372PurposeNucleocytoplasmic transport probeDepositorInsert(NLS)SV40-EGFP-6PrA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only