pCEP4-myc-PDGFRbeta
(Plasmid
#136455)
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PurposeMammalian expression plasmid for c-myc-tagged PDGFRB
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 136455 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepCEP4
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 10381
- Total vector size (bp) 13729
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePDGFRbeta
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Alt namePDGFRB
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Alt nameplatelet-derived growth factor receptor beta
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SpeciesH. sapiens (human)
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Insert Size (bp)3348
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GenBank IDNP_002600.1
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Entrez GenePDGFRB (a.k.a. CD140B, IBGC4, IMF1, JTK12, KOGS, PDGFR, PDGFR-1, PDGFR1, PENTT)
- Promoter CMV
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Tags
/ Fusion Proteins
- Signal peptide from influenza HA (N terminal on insert)
- Extracellular c-myc epitope tag
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer CMV-fwd
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCEP4-myc-PDGFRbeta was a gift from Erik Procko (Addgene plasmid # 136455 ; http://n2t.net/addgene:136455 ; RRID:Addgene_136455) -
For your References section:
Engineered receptors for human cytomegalovirus that are orthogonal to normal human biology. Park J, Gill KS, Aghajani AA, Heredia JD, Choi H, Oberstein A, Procko E. PLoS Pathog. 2020 Jun 19;16(6):e1008647. doi: 10.1371/journal.ppat.1008647. eCollection 2020 Jun. 10.1371/journal.ppat.1008647 PubMed 32559251