Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 11161 - 11180 of 14149 results

  5. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

    Plasmid
    #208645
    Purpose
    Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.
    Depositor
    Quanfu Ma
    Insert
    Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
    Use
    CRISPR; Ssdna donnor preparation for knock-in of…
    Tags
    Twin Strep
    Expression
    Mutation
    Two SNP (rs749555789, rs760194863) were introduce…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    208645-plasmid-map-sequence-id-415926

    Enlarge

  8. pJH4542

    Plasmid
    #200779
    Purpose
    Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune
    Depositor
    Mei Zhen
    Article
    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do
    Insert
    GCaMP6
    Use
    Tags
    mNeptune
    Expression
    Worm
    Mutation
    Promoter
    Ptwk-40
    Availability
    Academic Institutions and Nonprofits only
    200779-plasmid-map-sequence-id-412786

    Enlarge

  9. pYG205

    Plasmid
    #200836
    Purpose
    Transcribes rapZ from the arabinose-inducible PBAD promoter; RapZ directs RNase E to cleave fusion RNAs transcribed from pYG215 or derivatives
    Depositor
    Boris Görke
    Article
    Schilder et al Nucleic Acids Res. 2023 Mar 29:gkad226.
    Insert
    rapZ (rapZ Escherichia coli str. K-12 substr. MG1655)
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P-BAD
    Availability
    Academic Institutions and Nonprofits only
    200836-plasmid-map-sequence-id-404931

    Enlarge

  19. pICH-nuc-roGFP2-GPXC71S

    Plasmid
    #191760
    Purpose
    Ti plasmid pICH86988 harbouring nuc-roGFP2-GPXC71S -a nuclear-targeted control roGFP2 fusion not responsive to peroxides used alongside plasmids 191759 and 191758
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236
    Insert
    nuc-roGFP2-GPXC71S
    Use
    Binary agrobacterium ti plasmid
    Tags
    Expression
    Plant
    Mutation
    GPX has no peroxidatic Cysteine 71, which is repl…
    Promoter
    CaMV 35S
    Availability
    Academic Institutions and Nonprofits only
    191760-plasmid-map-sequence-id-378856

    Enlarge

Showing: 11161 - 11180 of 14149 results