Skip to main content
Addgene
Showing: 11161 - 11180 of 14801 results

  3. pJH4542

    Plasmid
    #200779
    Purpose
    Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune
    Depositor
    Mei Zhen
    Article
    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do
    Insert
    GCaMP6
    Use
    Tags
    mNeptune
    Expression
    Worm
    Mutation
    Promoter
    Ptwk-40
    Available since
    May 7, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    200779-plasmid-map-sequence-id-412786

    Enlarge

  5. p663-UBC-miniTurbo-V5-CDKL5_IDG-K

    Plasmid
    #170578
    Purpose
    Gateway destination clone of CDKL5 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    miniTurbo-V5-CDKL5 (CDKL5 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    miniTurbo-V5
    Expression
    Mammalian
    Mutation
    E854G- please see depositor comments
    Promoter
    Ubiquitin
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    170578-plasmid-map-sequence-id-340993

    Enlarge

  6. mEGFP-NbALFA

    Plasmid
    #159986
    Purpose
    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)
    Depositor
    Motoyuki Hattori
    Article
    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:
    Insert
    mEGFP-NbALFA
    Use
    Tags
    8histidine-HRV3C protease cleavage site
    Expression
    Bacterial
    Mutation
    3GS linker between mEGFP and NbALFA
    Promoter
    Available since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    159986-plasmid-map-sequence-id-314744

    Enlarge

  7. pET22b_PstS_1

    Plasmid
    #78198
    Purpose
    Expression of single-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensor
    Depositor
    Martin Webb
    Article
    Solscheid et al Biochemistry. 2015 Aug 18;54(32):5054-62
    Insert
    pstS (A197C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    A197C (please see depositors comment below)
    Promoter
    T7
    Available since
    July 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    78198-plasmid-map-sequence-id-146048

    Enlarge

  9. pJRoC30_VP8H

    Plasmid
    #188062
    Purpose
    PROSS-designed versatile peroxidase 8H
    Depositor
    Sarel Fleishman
    Article
    Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35
    Insert
    PROSS-designed versatile peroxidase from Ganoderma sp. 10597_SS1
    Use
    Tags
    S. cerevisiae native α-factor prepro-leader signa…
    Expression
    Yeast
    Mutation
    38 PROSS mutations, described in publication
    Promoter
    Available since
    Aug. 23, 2022
    Availability
    Academic Institutions and Nonprofits only
    188062-plasmid-map-sequence-id-370147

    Enlarge

  12. mCherry-NbALFA

    Plasmid
    #159987
    Purpose
    For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)
    Depositor
    Motoyuki Hattori
    Article
    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:
    Insert
    mCherry-NbALFA
    Use
    Tags
    8histidine-HRV3C protease cleavage site
    Expression
    Bacterial
    Mutation
    3GS linker between mCherry and NbALFA
    Promoter
    Available since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    159987-plasmid-map-sequence-id-314745

    Enlarge

  13. pSAG1::CAS9-U6::sgUPRT

    Plasmid
    #54467
    Purpose
    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for disruption of genes by CRISPR.
    Depositor
    David Sibley
    Article
    Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.
    Insert
    SAG1 5' UTR
    Use
    CRISPR; Toxoplasma expression
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    54467-plasmid-map-sequence-id-84808

    Enlarge

  15. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep

    Plasmid
    #68375
    Purpose
    Doyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.
    Depositor
    Yannick Doyon
    Article
    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and TALEN; Zfn
    Tags
    3xFLAG_Twin_Strep
    Expression
    Mammalian
    Mutation
    Promoter
    human PGK1
    Available since
    Oct. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    68375-plasmid-map-sequence-id-251912

    Enlarge

  20. pV92.2

    Plasmid
    #78409
    Purpose
    pKT230 plasmid engineered with the RTCas1v2-GFP cassette (RT-Cas1, Cas2, GFP) driven by the MMB-1 16S rRNA promoter, the CRISPR03 array driven by its native leader, and the tmRNA::td intron construct
    Depositor
    Andrew Fire
    Article
    Silas et al Science. 2016 Feb 26;351(6276):aad4234.
    Inserts
    RT-Cas1
    Cas2
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    78409-plasmid-map-sequence-id-146291

    Enlarge

Showing: 11161 - 11180 of 14801 results